The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

Details

Serval ID
serval:BIB_1923CE3245FD
Type
Article: article from journal or magazin.
Collection
Publications
Title
The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence
Journal
Sci Rep
Author(s)
Clamens T., Rosay T., Crepin A., Grandjean T., Kentache T., Hardouin J., Bortolotti P., Neidig A., Mooij M., Hillion M., Vieillard J., Cosette P., Overhage J., O'Gara F., Bouffartigues E., Dufour A., Chevalier S., Guery B., Cornelis P., Feuilloley M. G., Lesouhaitier O.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
2017
Volume
7
Pages
41178
Language
english
Notes
Clamens, Thomas
Rosay, Thibaut
Crepin, Alexandre
Grandjean, Teddy
Kentache, Takfarinas
Hardouin, Julie
Bortolotti, Perrine
Neidig, Anke
Mooij, Marlies
Hillion, Melanie
Vieillard, Julien
Cosette, Pascal
Overhage, Joerg
O'Gara, Fergal
Bouffartigues, Emeline
Dufour, Alain
Chevalier, Sylvie
Guery, Benoit
Cornelis, Pierre
Feuilloley, Marc G J
Lesouhaitier, Olivier
eng
Research Support, Non-U.S. Gov't
England
Sci Rep. 2017 Jan 24;7:41178. doi: 10.1038/srep41178.
Abstract
We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE(+)) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE(+) strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.
Keywords
Amidohydrolases/*metabolism, Animals, Biofilms, Caenorhabditis elegans/microbiology, Dictyostelium/microbiology, Female, Lung/microbiology, Male, Mice, Inbred C57BL, Proteome, Pseudomonas Infections/*enzymology/microbiology, Pseudomonas aeruginosa/*enzymology/*pathogenicity, Quorum Sensing, Virulence, *Virulence Factors
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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