Magnetic resonance-guided, real-time targeted delivery and imaging of magnetocapsules immunoprotecting pancreatic islet cells.

Details

Serval ID
serval:BIB_19165CA143D4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Magnetic resonance-guided, real-time targeted delivery and imaging of magnetocapsules immunoprotecting pancreatic islet cells.
Journal
Nature Medicine
Author(s)
Barnett B.P., Arepally A., Karmarkar P.V., Qian D., Gilson W.D., Walczak P., Howland V., Lawler L., Lauzon C., Stuber M., Kraitchman D.L., Bulte J.W.
ISSN
1078-8956[print], 1078-8956[linking]
Publication state
Published
Issued date
2007
Volume
13
Number
8
Pages
986-991
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Abstract
In type I diabetes mellitus, islet transplantation provides a moment-to-moment fine regulation of insulin. Success rates vary widely, however, necessitating suitable methods to monitor islet delivery, engraftment and survival. Here magnetic resonance-trackable magnetocapsules have been used simultaneously to immunoprotect pancreatic beta-cells and to monitor, non-invasively in real-time, hepatic delivery and engraftment by magnetic resonance imaging (MRI). Magnetocapsules were detected as single capsules with an altered magnetic resonance appearance on capsule rupture. Magnetocapsules were functional in vivo because mouse beta-cells restored normal glycemia in streptozotocin-induced diabetic mice and human islets induced sustained C-peptide levels in swine. In this large-animal model, magnetocapsules could be precisely targeted for infusion by using magnetic resonance fluoroscopy, whereas MRI facilitated monitoring of liver engraftment over time. These findings are directly applicable to ongoing improvements in islet cell transplantation for human diabetes, particularly because our magnetocapsules comprise clinically applicable materials.
Keywords
Animals, Capsules, Cell Survival/drug effects, Cells, Cultured, Diabetes Mellitus, Type 1/surgery, Drug Delivery Systems, Humans, Immunosuppressive Agents/administration & dosage, Immunosuppressive Agents/pharmacology, Islets of Langerhans/cytology, Islets of Langerhans/drug effects, Islets of Langerhans Transplantation, Magnetic Resonance Imaging/methods, Magnetics, Mice, Swine, Time Factors
Pubmed
Web of science
Create date
02/03/2010 16:04
Last modification date
20/08/2019 12:49
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