A bioactive injectable bulking material; a potential therapeutic approach for stress urinary incontinence.

Details

Serval ID
serval:BIB_18FE4C730F63
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A bioactive injectable bulking material; a potential therapeutic approach for stress urinary incontinence.
Journal
Biomaterials
Author(s)
Vardar E., Vythilingam G., Pinnagoda K., Engelhardt E.M., Zambelli P.Y., Hubbell J.A., Lutolf M.P., Frey P., Larsson H.M.
ISSN
1878-5905 (Electronic)
ISSN-L
0142-9612
Publication state
Published
Issued date
06/2019
Peer-reviewed
Oui
Volume
206
Pages
41-48
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Stress urinary incontinence (SUI) is a life changing condition, affecting 20 million women worldwide. In this study, we developed a bioactive, injectable bulking agent that consists of Permacol™ (Medtronic, Switzerland) and recombinant insulin like growth factor-1 conjugated fibrin micro-beads (fib_rIGF-1) for its bulk stability and capacity to induce muscle regeneration. Therefore, Permacol™ formulations were injected in the submucosal space of rabbit bladders. The ability of a bulking material to form a stable and muscle-inducing bulk represents for us a promising therapeutic approach to achieve a long-lasting treatment for SUI. The fib_rIGF-1 showed no adverse effect on human smooth muscle cell metabolic activity and viability in vitro based on AlamarBlue assays and Live/Dead staining. Three months after injection of fib_rIGF-1 together with Permacol™ into the rabbit bladder wall, we observed a smooth muscle tissue like formation within the injected materials. Positive staining for alpha smooth muscle actin, calponin, and caldesmon demonstrated a contractile phenotype of the newly formed smooth muscle tissue. Moreover, the fib_rIGF-1 treated group also improved the neovascularization at the injection site, confirmed by CD31 positive staining compared to bulks made of Permacol <sup>TM</sup> only. The results of this study encourage us to further develop this injectable, bioactive bulking material towards a future therapeutic approach for a minimal invasive and long-lasting treatment of SUI.
Keywords
Fibrin micro-beads, Rabbit bladder, Recombinant insulin-like growth factor-1, Stress urinary incontinence
Pubmed
Create date
15/04/2019 10:11
Last modification date
20/08/2019 13:49
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