Wharton's jelly mesenchymal stromal/stem cells derived under chemically defined animal product-free low oxygen conditions are rich in MSCA-1(+) subpopulation.
Details
Serval ID
serval:BIB_18E678727078
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Wharton's jelly mesenchymal stromal/stem cells derived under chemically defined animal product-free low oxygen conditions are rich in MSCA-1(+) subpopulation.
Journal
Regenerative medicine
ISSN
1746-076X (Electronic)
ISSN-L
1746-0751
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
9
Number
6
Pages
723-732
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Umbilical cord contains, within Wharton's jelly (WJ), multipotent mesenchymal stromal/stem cells (MSCs) of fetal origin that can be isolated and expanded in vitro with a minimal manipulation and very high efficiency. Our aim was to develop a highly reproducible protocol that has the unique potential to be scaled up and adapted to cGMP requirements for the use in cellular therapy.
We found that derivation of WJ MSCs under defined conditions in low oxygen resulted in several folds higher populations of MSCA-1(+) cells (6.0-19.2%) when compared with WJ MSCs derived in the presence of serum (0.1-2.8%) or clinical-grade bone marrow (BM) MSCs cultured under atmospheric O2 (20%). We demonstrate that WJ MSCs derived following our protocol display antiproliferative activity similar to clinical-grade BM MSCs. We also show that these WJ MSCs can be differentiated into adipo-, chondro- and osteo-genic lineages.
Easy accessibility, abundance and genetic 'naivety' make WJ MSCs logistically a more attractive source for clinical applications than BM MSCs.
We found that derivation of WJ MSCs under defined conditions in low oxygen resulted in several folds higher populations of MSCA-1(+) cells (6.0-19.2%) when compared with WJ MSCs derived in the presence of serum (0.1-2.8%) or clinical-grade bone marrow (BM) MSCs cultured under atmospheric O2 (20%). We demonstrate that WJ MSCs derived following our protocol display antiproliferative activity similar to clinical-grade BM MSCs. We also show that these WJ MSCs can be differentiated into adipo-, chondro- and osteo-genic lineages.
Easy accessibility, abundance and genetic 'naivety' make WJ MSCs logistically a more attractive source for clinical applications than BM MSCs.
Keywords
Cell Culture Techniques/methods, Cell Differentiation, Cell Proliferation, Cells, Cultured, Female, Flow Cytometry, Humans, Mesenchymal Stem Cells/cytology, Oxygen/metabolism, Serum/chemistry, Umbilical Cord/cytology, Wharton Jelly/cytology, MSCA-1, Wharton's jelly, animal product-free culture, chemically defined condition, mesenchymal stem cell, mesenchymal stromal cell, umbilical cord
Pubmed
Web of science
Create date
12/01/2024 10:14
Last modification date
13/01/2024 7:10
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