Fiber density of collagen grafts impacts rabbit urethral regeneration.
Details
Serval ID
serval:BIB_18BB59E93239
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fiber density of collagen grafts impacts rabbit urethral regeneration.
Journal
Scientific reports
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
03/07/2018
Peer-reviewed
Oui
Volume
8
Number
1
Pages
10057
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Abstract
There is a need for efficient and "off-the-shelf" grafts in urethral reconstructive surgery. Currently available surgical techniques require harvesting of grafts from autologous sites, with increased risk of surgical complications and added patient discomfort. Therefore, a cost-effective and cell-free graft with adequate regenerative potential has a great chance to be translated into clinical practice. Tubular cell-free collagen grafts were prepared by varying the collagen density and fiber distribution, thereby creating a polarized low fiber density collagen graft (LD-graft). A uniform, high fiber density collagen graft (HD-graft) was engineered as a control. These two grafts were implanted to bridge a 2 cm long iatrogenic urethral defect in a rabbit model. Histology revealed that rabbits implanted with the LD-graft had a better smooth muscle regeneration compared to the HD-graft. The overall functional outcome assessed by contrast voiding cystourethrography showed patency of the urethra in 90% for the LD-graft and in 66.6% for the HD-graft. Functional regeneration of the rabbit implanted with the LD-graft could further be demonstrated by successful mating, resulting in healthy offspring. In conclusion, cell-free low-density polarized collagen grafts show better urethral regeneration than high-density collagen grafts.
Keywords
Animals, Collagen/metabolism, Dietary Fiber, Extracellular Matrix, Male, Models, Animal, Muscle, Smooth, Rabbits, Reconstructive Surgical Procedures, Regeneration, Tissue Engineering/methods, Transplants/metabolism, Transplants/surgery, Urethra/pathology, Urethra/transplantation
Pubmed
Web of science
Open Access
Yes
Create date
29/07/2018 13:14
Last modification date
30/04/2021 7:08