Fiber density of collagen grafts impacts rabbit urethral regeneration.

Details

Serval ID
serval:BIB_18BB59E93239
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fiber density of collagen grafts impacts rabbit urethral regeneration.
Journal
Scientific reports
Author(s)
Larsson H.M., Vythilingam G., Pinnagoda K., Vardar E., Engelhardt E.M., Sothilingam S., Thambidorai R.C., Kamarul T., Hubbell J.A., Frey P.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
03/07/2018
Peer-reviewed
Oui
Volume
8
Number
1
Pages
10057
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
There is a need for efficient and "off-the-shelf" grafts in urethral reconstructive surgery. Currently available surgical techniques require harvesting of grafts from autologous sites, with increased risk of surgical complications and added patient discomfort. Therefore, a cost-effective and cell-free graft with adequate regenerative potential has a great chance to be translated into clinical practice. Tubular cell-free collagen grafts were prepared by varying the collagen density and fiber distribution, thereby creating a polarized low fiber density collagen graft (LD-graft). A uniform, high fiber density collagen graft (HD-graft) was engineered as a control. These two grafts were implanted to bridge a 2 cm long iatrogenic urethral defect in a rabbit model. Histology revealed that rabbits implanted with the LD-graft had a better smooth muscle regeneration compared to the HD-graft. The overall functional outcome assessed by contrast voiding cystourethrography showed patency of the urethra in 90% for the LD-graft and in 66.6% for the HD-graft. Functional regeneration of the rabbit implanted with the LD-graft could further be demonstrated by successful mating, resulting in healthy offspring. In conclusion, cell-free low-density polarized collagen grafts show better urethral regeneration than high-density collagen grafts.
Keywords
Animals, Collagen/metabolism, Dietary Fiber, Extracellular Matrix, Male, Models, Animal, Muscle, Smooth, Rabbits, Reconstructive Surgical Procedures, Regeneration, Tissue Engineering/methods, Transplants/metabolism, Transplants/surgery, Urethra/pathology, Urethra/transplantation
Pubmed
Web of science
Open Access
Yes
Create date
29/07/2018 13:14
Last modification date
16/12/2019 7:19
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