Loss of connexin40 is associated with decreased endothelium-dependent relaxations and eNOS levels in the mouse aorta.

Details

Serval ID
serval:BIB_188385A51A19
Type
Article: article from journal or magazin.
Collection
Publications
Title
Loss of connexin40 is associated with decreased endothelium-dependent relaxations and eNOS levels in the mouse aorta.
Journal
American Journal of Physiology. Heart and Circulatory Physiology
Author(s)
Alonso Florian, Boittin Francois-Xavier, Beny Jean-Louis, Haefliger Jacques-Antoine
ISSN
1522-1539[electronic], 0363-6135[linking]
Publication state
Published
Issued date
2010
Volume
299
Number
5
Pages
1365-1373
Language
english
Abstract
Upon agonist stimulation, endothelial cells trigger smooth muscle relaxation through the release of relaxing factors such as nitric oxide (NO). Endothelial cells of mouse aorta are interconnected by gap junctions made of connexin40 (Cx40) and connexin37 (Cx37), allowing the exchange of signaling molecules to coordinate their activity. Wild-type (Cx40(+/+)) and hypertensive Cx40-deficient mice (Cx40(-/-)), which also exhibit a marked decrease of Cx37 in the endothelium, were used to investigate the link between the expression of endothelial connexins (Cx40 and Cx37) and endothelial nitric oxide synthase (eNOS) expression and function in the mouse aorta. With the use of isometric tension measurements in aortic rings precontracted with U-46619, a stable thromboxane A(2) mimetic, we first demonstrate that ACh- and ATP-induced endothelium-dependent relaxations solely depend on NO release in both Cx40(+/+) and Cx40(-/-) mice, but are markedly weaker in Cx40(-/-) mice. Consistently, both basal and ACh- or ATP-induced NO production were decreased in the aorta of Cx40(-/-) mice. Altered relaxations and NO release from aorta of Cx40(-/-) mice were associated with lower expression levels of eNOS in the aortic endothelium of Cx40(-/-) mice. Using immunoprecipitation and in situ ligation assay, we further demonstrate that eNOS, Cx40, and Cx37 tightly interact with each other at intercellular junctions in the aortic endothelium of Cx40(+/+) mice, suggesting that the absence of Cx40 in association with altered Cx37 levels in endothelial cells from Cx40(-/-) mice participate to the decreased levels of eNOS. Altogether, our data suggest that the endothelial connexins may participate in the control of eNOS expression levels and function.
Keywords
Connexin40, Connexin37, Endothelium-Dependent Relaxation, Endothelial Nitric Oxide Synthase, Nitric-Oxide Synthase, Gap-Junctions, Functional Expression, Proximity Ligation, Hypertensive-Rats, Down-Regulation, Angiotensin-Ii, Knockout Mice, Up-Regulation, In-Situ
Pubmed
Web of science
Create date
09/12/2010 12:54
Last modification date
20/08/2019 13:49
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