Individuality of Ag-selected and preimmune TCR repertoires.

Details

Serval ID
serval:BIB_18771
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Individuality of Ag-selected and preimmune TCR repertoires.
Journal
Immunologic Research
Author(s)
Maryanski J.L., Attuil V., Hamrouni A., Mutin M., Rossi M., Aublin A., Bucher P.
ISSN
0257-277X
Publication state
Published
Issued date
2001
Volume
23
Number
1
Pages
75-84
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review Publication Status: ppublish
Abstract
We have combined flow cytometry and single-cell PCR to characterize the TCRBV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW3 170-1 79/Kd). Ourresults established thatdifferent mice select individually distinct yet structurally similar CW3-specific repertoires. Repertoire selection appears to be flexible depending on the immunizing cell dose. Using a single-donor, matched-pair-recipient adoptive transfer strategy, we demonstrated that the CW3-specific TCR repertoires of normal mice are already distinct at the preimmune level. We combine our data with computer simulations to test models for the composition of an Ag-specific preimmune repertoire and its selection during an immune response.
Keywords
Animals, CD8-Positive T-Lymphocytes/immunology, Computer Simulation, Flow Cytometry, Gene Library, HLA-C Antigens/immunology, Humans, Mice, Polymerase Chain Reaction, Receptors, Antigen, T-Cell, alpha-beta/immunology
Pubmed
Web of science
Create date
19/11/2007 9:40
Last modification date
20/08/2019 12:48
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