Quantitative real-time polymerase chain reaction for detection of adenovirus after T cell-replete hematopoietic cell transplantation: viral load as a marker for invasive disease.

Details

Serval ID
serval:BIB_182D07CE93BA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Quantitative real-time polymerase chain reaction for detection of adenovirus after T cell-replete hematopoietic cell transplantation: viral load as a marker for invasive disease.
Journal
Clinical Infectious Diseases
Author(s)
Erard Véronique, Huang Meei-Li, Ferrenberg James, Nguy Long, Stevens-Ayers Terry L., Hackman Robert C., Corey Lawrence, Boeckh Michael
ISSN
1537-6591[electronic]
Publication state
Published
Issued date
2007
Volume
45
Number
8
Pages
958-965
Language
english
Abstract
BACKGROUND: The value of adenovirus plasma DNA detection as an indicator for adenovirus disease is unknown in the context of T cell-replete hematopoietic cell transplantation, of which adenovirus disease is an uncommon but serious complication. METHODS: Three groups of 62 T cell-replete hematopoietic cell transplant recipients were selected and tested for adenovirus in plasma by polymerase chain reaction. RESULTS: Adenovirus was detected in 21 (87.5%) of 24 patients with proven adenovirus disease (group 1), in 4 (21%) of 19 patients who shed adenovirus (group 2), and in 1 (10.5%) of 19 uninfected control patients. The maximum viral load was significantly higher in group 1 (median maximum viral load, 6.3x10(6) copies/mL; range, 0 to 1.0x10(9) copies/mL) than in group 2 (median maximum viral load, 0 copies/mL; range, 0 to 1.7x10(8) copies/mL; P<.001) and in group 3 (median maximum viral load, 0 copies/mL; range 0-40 copies/mL; P<.001). All patients in group 2 who developed adenoviremia had symptoms compatible with adenovirus disease (i.e., possible disease). A minimal plasma viral load of 10(3) copies/mL was detected in all patients with proven or possible disease. Adenoviremia was detectable at a median of 19.5 days (range, 8-48 days) and 24 days (range, 9-41 days) before death for patients with proven and possible adenovirus disease, respectively. CONCLUSION: Sustained or high-level adenoviremia appears to be a specific and sensitive indicator of adenovirus disease after T cell-replete hematopoietic cell transplantation. In the context of low prevalence of adenovirus disease, the use of polymerase chain reaction of plasma specimens to detect virus might be a valuable tool to identify and treat patients at risk for viral invasive disease.
Keywords
Adenovirus Infections, Human/virology, Adenoviruses, Human/genetics, Adenoviruses, Human/isolation & purification, Adolescent, Adult, Child, Child, Preschool, DNA, Viral/blood, Hematologic Diseases/complications, Hematopoietic Stem Cell Transplantation/adverse effects, Humans, Middle Aged, Polymerase Chain Reaction/methods, Predictive Value of Tests, Time Factors, Viral Load
Pubmed
Web of science
Open Access
Yes
Create date
02/02/2010 16:22
Last modification date
20/08/2019 13:48
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