Tissue-specific segregation of TCRgamma delta+ NKT cells according to phenotype TCR repertoire and activation status: parallels with TCR alphabeta+NKT cells.

Details

Serval ID
serval:BIB_18291
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tissue-specific segregation of TCRgamma delta+ NKT cells according to phenotype TCR repertoire and activation status: parallels with TCR alphabeta+NKT cells.
Journal
European Journal of Immunology
Author(s)
Lees R.K., Ferrero I., MacDonald H.R.
ISSN
0014-2980
Publication state
Published
Issued date
2001
Volume
31
Number
10
Pages
2901-2909
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
Whereas the majority of NKT cells in the mouse express an alpha beta TCR (NKTalpha beta cells), a small subset of NKT cells express a gamma delta TCR (NKTgamma delta). Here we have systematically analyzed the phenotype, TCR repertoire and activation status of NKTgamma delta cells in the thymus, liver, spleen and bone marrow of normal C57BL/6 mice. Our data indicate that NKTgamma delta cells segregate in a tissue-specific manner according to these parameters. While most NKTgamma delta cells in the thymus and liver have a recently activated CD62L(lo) phenotype and a TCR repertoire that is heavily biased to Vgamma1.1 and Vdelta6.3, the majority of NKTgamma delta cells in the spleen and bone marrow are CD62L(hi) and have a much less biased TCR repertoire. Moreover, expression of NK markers is high on NKTgamma delta cells in spleen and bone marrow but low in thymus and liver. Collectively our results reveal a tissue-specific segregation of NKTgamma delta cells that is strikingly similar to that recently described for CD1d-dependent and Cd1d-independent NKTalpha beta cells. We therefore propose that chronic TCR activation by tissue-specific endogenous ligands is a generic property of NKT cells of both the alpha beta and gamma delta lineages.
Keywords
Animals, Antigens, CD4/analysis, Antigens, CD8/analysis, Antigens, Ly, Female, Immunophenotyping, Killer Cells, Natural/physiology, Lectins, C-Type, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Organ Specificity, Receptors, Antigen, T-Cell, alpha-beta/analysis, Receptors, Antigen, T-Cell, gamma-delta/analysis, Receptors, Immunologic/analysis, Receptors, NK Cell Lectin-Like
Pubmed
Web of science
Create date
19/11/2007 12:12
Last modification date
20/08/2019 12:48
Usage data