Stapled Porcine Pericardium Displays Lower Infectivity In Vitro Than Native and Sutured Porcine Pericardium.

Details

Serval ID
serval:BIB_17ED46F3E2BE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stapled Porcine Pericardium Displays Lower Infectivity In Vitro Than Native and Sutured Porcine Pericardium.
Journal
The Journal of surgical research
Author(s)
Del Tatto B., Le Roy D., Lambelet M., Corpataux J.M., Chakfé N., Giulieri S., Allagnat F., Roger T., Saucy F.
ISSN
1095-8673 (Electronic)
ISSN-L
0022-4804
Publication state
Published
Issued date
04/2022
Peer-reviewed
Oui
Volume
272
Pages
132-138
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Biological xenografts using tubulized porcine pericardium are an alternative to replace infected prosthetic graft. We recently reported an innovative technique using a stapled porcine pericardial bioconduit for immediate vascular reconstruction in emergency. The objective of this study is to compare the growth and adherence to grafts of bacteria and yeast incubated with stapled porcine pericardium, sutured or naked pericardium.
One square centimeter of porcine pericardial patches, with or without staples or sutures, was incubated with 10 <sup>5</sup> colony forming units of Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans for 1, 6, and 24 h. The medium was collected to quantify planktonic microorganisms, while grafts were sonicated to quantify adherent microorganisms. Dacron and Dacron Silver were analyzed in parallel as synthetic reference prostheses.
Stapled porcine pericardium reduced the growth and the adherence of E coli (2- to 30-fold; P < 0.0005), S aureus (11- to 1000-fold; P < 0.0006), S epidermidis (>500-fold; P < 0.0001), and C albicans (12- to 50-fold; P < 0.0001) when compared to medium alone (growth) and pericardium or Dacron (adherence). Native and sutured porcine pericardium interfered with the growth and the adherence of E coli and C albicans, and Dacron with that of S epidermidis. As expected, Dacron Silver was robustly bactericidal.
Stapled porcine pericardium exhibited a lower susceptibility to infection by bacteria and yeasts in vitro when compared to the native and sutured porcine pericardium. Stapled porcine pericardium might be a good option for rapid vascular grafting without increasing infectivity.
Keywords
Adherence, Dacron, Infection, Pericardium, Vascular graft
Pubmed
Web of science
Funding(s)
Swiss National Science Foundation / Projects / 310030_173123
Swiss National Science Foundation / Projects / 310030_176158
Create date
21/01/2022 18:15
Last modification date
01/04/2022 6:34
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