Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.

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Serval ID
serval:BIB_17DFDA5C12C3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.
Journal
Nucleic Acids Research
Author(s)
Orioli A., Pascali C., Quartararo J., Diebel K.W., Praz V., Romascano D., Percudani R., van Dyk L.F., Hernandez N., Teichmann M., Dieci G.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
2011
Volume
39
Number
13
Pages
5499-5512
Language
english
Abstract
Human RNA polymerase (Pol) III-transcribed genes are thought to share a simple termination signal constituted by four or more consecutive thymidine residues in the coding DNA strand, just downstream of the RNA 3'-end sequence. We found that a large set of human tRNA genes (tDNAs) do not display any T(≥4) stretch within 50 bp of 3'-flanking region. In vitro analysis of tDNAs with a distanced T(≥4) revealed the existence of non-canonical terminators resembling degenerate T(≥5) elements, which ensure significant termination but at the same time allow for the production of Pol III read-through pre-tRNAs with unusually long 3' trailers. A panel of such non-canonical signals was found to direct transcription termination of unusual Pol III-synthesized viral pre-miRNA transcripts in gammaherpesvirus 68-infected cells. Genome-wide location analysis revealed that human Pol III tends to trespass into the 3'-flanking regions of tDNAs, as expected from extensive terminator read-through. The widespread occurrence of partial termination suggests that the Pol III primary transcriptome in mammals is unexpectedly enriched in 3'-trailer sequences with the potential to contribute novel functional ncRNAs.
Keywords
3' Flanking Region, Animals, Cell Line, Hela Cells, Humans, Mice, RNA Polymerase III/metabolism, RNA, Transfer/genetics, Sequence Analysis, DNA, Terminator Regions, Genetic, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
28/03/2011 11:15
Last modification date
20/08/2019 13:47
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