Gene expression profiling identifies emerging oncogenic pathways operating in extranodal NK/T-cell lymphoma, nasal type.

Details

Serval ID
serval:BIB_17A96CCBC0E5
Type
Article: article from journal or magazin.
Collection
Publications
Title
Gene expression profiling identifies emerging oncogenic pathways operating in extranodal NK/T-cell lymphoma, nasal type.
Journal
Blood
Author(s)
Huang Y., de Reyniès A., de Leval L., Ghazi B., Martin-Garcia N., Travert M., Bosq J., Brière J., Petit B., Thomas E., Coppo P., Marafioti T., Emile J.F., Delfau-Larue M.H., Schmitt C., Gaulard P.
ISSN
1528-0020[electronic], 0006-4971[linking]
Publication state
Published
Issued date
2010
Volume
115
Number
6
Pages
1226-1237
Language
english
Abstract
Biopsies and cell lines of natural killer/T-cell lymphoma, nasal type (NKTCL) were subject to combined gene expression profiling and array-based comparative genomic hybridization analyses. Compared with peripheral T-cell lymphoma, not otherwise specified, NKTCL had greater transcript levels for NK-cell and cytotoxic molecules, especially granzyme H. Compared with normal NKcells, tumors were closer to activated than resting cells and overexpressed several genes related to vascular biology, Epstein-Barr Virus-induced genes, and PDGFRA. Notably, platelet-derived growth factor receptor alpha and its phosphorylated form were confirmed at the protein level, and in vitro the MEC04 NKTCL cell line was sensitive to imatinib. Deregulation of the AKT, Janus kinase-signal transducers and activators of transcription, and nuclear factor-kappaB pathways was corroborated by nuclear expression of phosphorylated AKT, signal transducers and activators of transcription 3, and RelA in NKTCL, and several deregulated genes in these pathways mapped to regions of recurrent copy number aberrations (AKT3 [1q44], IL6R [1q21.3], CCL2 [17q12], TNFRSF21 [6p12.3]). Several features of NKTCL uncovered by this analysis suggest perturbation of angiogenic pathways. Integrative analysis also evidenced deregulation of the tumor suppressor HACE1 in the frequently deleted 6q21 region. This study highlights emerging oncogenic pathways in NKTCL and identifies novel diagnostic and therapeutic targets.
Keywords
Adult, Aged, Blotting, Western, Cell Proliferation, Cells, Cultured, Comparative Genomic Hybridization, Epstein-Barr Virus Infections/genetics, Epstein-Barr Virus Infections/metabolism, Female, Gene Expression Profiling, Herpesvirus 4, Human/physiology, Humans, Immunoenzyme Techniques, Killer Cells, Natural/pathology, Killer Cells, Natural/virology, Lymphoma, Extranodal NK-T-Cell/genetics, Lymphoma, Extranodal NK-T-Cell/metabolism, Male, Middle Aged, Nasal Mucosa/metabolism, Nasal Mucosa/pathology, Nasopharyngeal Neoplasms/genetics, Nasopharyngeal Neoplasms/metabolism, Oligonucleotide Array Sequence Analysis, Oncogenes/physiology, RNA, Messenger/genetics, RNA, Messenger/metabolism, Receptors, Platelet-Derived Growth Factor/genetics, Receptors, Platelet-Derived Growth Factor/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Tumor Markers, Biological/genetics, Tumor Markers, Biological/metabolism, Ubiquitin-Protein Ligases/genetics, Ubiquitin-Protein Ligases/metabolism
Pubmed
Open Access
Yes
Create date
22/10/2010 9:51
Last modification date
20/08/2019 12:47
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