Sex-biased microRNA expression in mammals and birds reveals underlying regulatory mechanisms and a role in dosage compensation.

Details

Serval ID
serval:BIB_17512FD3E952
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sex-biased microRNA expression in mammals and birds reveals underlying regulatory mechanisms and a role in dosage compensation.
Journal
Genome research
Author(s)
Warnefors M., Mössinger K., Halbert J., Studer T., VandeBerg J.L., Lindgren I., Fallahshahroudi A., Jensen P., Kaessmann H.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Publication state
Published
Issued date
12/2017
Peer-reviewed
Oui
Volume
27
Number
12
Pages
1961-1973
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Sexual dimorphism depends on sex-biased gene expression, but the contributions of microRNAs (miRNAs) have not been globally assessed. We therefore produced an extensive small RNA sequencing data set to analyze male and female miRNA expression profiles in mouse, opossum, and chicken. Our analyses uncovered numerous cases of somatic sex-biased miRNA expression, with the largest proportion found in the mouse heart and liver. Sex-biased expression is explained by miRNA-specific regulation, including sex-biased chromatin accessibility at promoters, rather than piggybacking of intronic miRNAs on sex-biased protein-coding genes. In mouse, but not opossum and chicken, sex bias is coordinated across tissues such that autosomal testis-biased miRNAs tend to be somatically male-biased, whereas autosomal ovary-biased miRNAs are female-biased, possibly due to broad hormonal control. In chicken, which has a Z/W sex chromosome system, expression output of genes on the Z Chromosome is expected to be male-biased, since there is no global dosage compensation mechanism that restores expression in ZW females after almost all genes on the W Chromosome decayed. Nevertheless, we found that the dominant liver miRNA, miR-122-5p, is Z-linked but expressed in an unbiased manner, due to the unusual retention of a W-linked copy. Another Z-linked miRNA, the male-biased miR-2954-3p, shows conserved preference for dosage-sensitive genes on the Z Chromosome, based on computational and experimental data from chicken and zebra finch, and acts to equalize male-to-female expression ratios of its targets. Unexpectedly, our findings thus establish miRNA regulation as a novel gene-specific dosage compensation mechanism.
Keywords
Animals, Chickens/genetics, Datasets as Topic, Dosage Compensation, Genetic/genetics, Female, Finches/genetics, Gene Expression Profiling, Male, Mice, MicroRNAs/biosynthesis, MicroRNAs/genetics, Monodelphis/genetics, Proteins/genetics, Regulatory Sequences, Nucleic Acid, Sex Characteristics
Pubmed
Web of science
Open Access
Yes
Create date
02/01/2018 16:42
Last modification date
20/08/2019 12:47
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