Human liver carboxylesterases: purification of these two enzymes, properties in relation to cocaine and alcohol metabolism, and specificities.

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State: Public
Version: author
Serval ID
serval:BIB_17382
Type
Inproceedings: an article in a conference proceedings.
Collection
Publications
Title
Human liver carboxylesterases: purification of these two enzymes, properties in relation to cocaine and alcohol metabolism, and specificities.
Title of the conference
37th Tiaft Trienna Meeting
Author(s)
Berthoin K, Widmer F, Giroud C, Mangin P, Barret L
Address
Cracow, Kraków, September 5-9, 1999
ISBN
1230-7483
ISSN-L
1230-7483
Publication state
Published
Issued date
2000
Peer-reviewed
Oui
Volume
43
Series
Problems of forensic Sciences
Pages
35-42
Language
english
Abstract
The purpose of this work was: 1) investigation of cocaine metabolism in association with alcohol intake thoroughly. Carboxylesterases specificities with cocaine and cocaethylene metabolites were determined by incubating one of the two purified enzymes with benzoylecgonine, ecgonine methyl ester, ecgonine ethyl ester, norcocaine and norcocaethylene;
2) determination of each enzyme affinity for various xenobiotics. The first step consisted in isolating carboxylesterases 1 and 2 from human liver obtained by autopsy
Keywords
Cocaine, Ethanol, Cocaethylene, Carboxylesterases
Create date
19/11/2007 12:11
Last modification date
20/08/2019 12:47
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