Automated Incubation and Digital Image Analysis of Chromogenic Media Using Copan WASPLab Enables Rapid Detection of Vancomycin-Resistant Enterococcus.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_16A1ACE00AF2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Automated Incubation and Digital Image Analysis of Chromogenic Media Using Copan WASPLab Enables Rapid Detection of Vancomycin-Resistant Enterococcus.
Journal
Frontiers in cellular and infection microbiology
Author(s)
Cherkaoui A., Renzi G., Charretier Y., Blanc D.S., Vuilleumier N., Schrenzel J.
ISSN
2235-2988 (Electronic)
ISSN-L
2235-2988
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
9
Pages
379
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Objective: The aim of the present study was to assess whether the WASPLab automation enables faster detection of vancomycin-resistant Enterococcus (VRE) on chromogenic VRE-specific plates by shortening the incubation time. Methods: Ninety different VRE culture negative rectal ESwab specimens were spiked with various concentrations (ranging from 3 × 10 <sup>2</sup> to 3 × 10 <sup>7</sup> CFU/ml) of 10 Enterococcus faecium strains (vancomycin MICs ranging from 32 to >256 mg/l), 3 E. faecium VanB strains (vancomycin MICs: 4, 8, and 16 mg/l), and 2 E. faecium VanB strains displaying vancomycin heteroresistance (vancomycin MICs: 64 and 96 mg/l). Results: Besides the two strains exhibiting vancomycin heteroresistance, all the other 13 VRE strains included in this study were detected as early as 24 h on the WASPLab even if the inoculum was low (3 × 10 <sup>3</sup> CFU/ml). When the vancomycin MICs were high, all strains were detected as early as at 18 h. However, 30 h was a conservative time point for finalizing the analysis of chromogenic cultures. Conclusion: These results suggested that the WASPLab automated incubation could allow decreasing the initial incubation time to 18 h, followed by an intermediate time at 24 h and a final incubation period of 30 h for VRE culture screening, to deliver rapid results without affecting the analytical sensitivity.
Keywords
Anti-Bacterial Agents/pharmacology, Bacteriological Techniques, Gram-Positive Bacterial Infections/diagnosis, Gram-Positive Bacterial Infections/microbiology, Humans, Microbial Sensitivity Tests/methods, Molecular Imaging/methods, Sensitivity and Specificity, Vancomycin-Resistant Enterococci/drug effects, Vancomycin-Resistant Enterococci/metabolism, Copan WASPLab, VRE, chromogenic media, culture, screening, time to results, vancomycin-resistant Enterococcus
Pubmed
Web of science
Open Access
Yes
Create date
07/01/2020 12:50
Last modification date
25/07/2020 6:19
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