Comparative T cell receptor repertoire selection by antigen after adoptive transfer: a glimpse at an antigen-specific preimmune repertoire.

Details

Serval ID
serval:BIB_16739
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparative T cell receptor repertoire selection by antigen after adoptive transfer: a glimpse at an antigen-specific preimmune repertoire.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Attuil V., Bucher P., Rossi M., Mutin M., Maryanski J.L.
ISSN
0027-8424
Publication state
Published
Issued date
2000
Volume
97
Number
15
Pages
8473-8478
Language
english
Abstract
The low frequency of precursor cells specific for any particular antigen (Ag) makes it difficult to characterize preimmune T cell receptor (TCR) repertoires and to understand repertoire selection during an immune response. We have undertaken a combined adoptive transfer single-cell PCR approach to probe the Ag-specific preimmune repertoires of individual mice. Our strategy was to inject paired irradiated recipient mice with normal spleen cells prepared from individual donors and to compare the TCR repertoires subsequently selected during a CD8 response to a defined model Ag. We found that although some TCRs were shared, the TCR repertoires selected by mice receiving splenocytes from the same donor were not identical in terms of the TCRs selected and their relative frequencies. Our results together with computer simulations imply that individual mice express distinct Ag-specific preimmune TCR repertoires composed of expanded clones and that selection by Ag is a random process.
Keywords
Adoptive Transfer/methods, Animals, Base Sequence, CD8-Positive T-Lymphocytes/immunology, DNA, Complementary, Female, Gene Rearrangement, T-Lymphocyte, HLA-C Antigens/immunology, L-Selectin/immunology, Mice, Mice, Inbred DBA, Molecular Sequence Data, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology, Spleen/cytology, Spleen/immunology
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 9:38
Last modification date
20/08/2019 12:46
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