Myc induces the nucleolin and BN51 genes: possible implications in ribosome biogenesis.

Details

Serval ID
serval:BIB_16736
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Myc induces the nucleolin and BN51 genes: possible implications in ribosome biogenesis.
Journal
Nucleic Acids Research
Author(s)
Greasley P.J., Bonnard C., Amati B.
ISSN
1362-4962[electronic]
Publication state
Published
Issued date
2000
Volume
28
Number
2
Pages
446-453
Language
english
Notes
Publication types: Journal Article
Abstract
The c-Myc oncoprotein and its dimerization partner Max bind the DNA core consensus sequence CACGTG (E-box) and activate gene transcription. However, the low levels of induction have hindered the identification of novel Myc target genes by differential screening techniques. Here, we describe a computer-based pre-selection of candidate Myc/Max target genes, based on two restrictive criteria: an extended E-box consensus sequence for Myc/Max binding and the occurrence of this sequence within a potential genomic CpG island. Candidate genes selected by these criteria were evaluated experimentally for their response to Myc. Two Myc target genes are characterized here in detail. These encode nucleolin, an abundant nucleolar protein, and BN51, a co-factor of RNA polymerase III. Myc activates transcription of both genes via E-boxes located in their first introns, as seen for several well-characterized Myc targets. For both genes, mutation of the E-boxes abolishes transcriptional activation by Myc as well as repression by Mad1. In addition, the BN51 promoter is selectively activated by Myc and not by USF, another E-box-binding factor. Both nucleolin and BN51 are implicated in the maturation of ribosomal RNAs, albeit in different ways. We propose that Myc, via regulation of these and probably many other transcriptional targets, may be an important regulator of ribosome biogenesis.
Keywords
Cell Cycle Proteins/genetics, Cell Line, Humans, Molecular Sequence Data, Neoplasm Proteins, Phosphoproteins/genetics, Plasmids, Proto-Oncogene Proteins c-myc/physiology, RNA Polymerase III/genetics, RNA-Binding Proteins/genetics, Ribosomes/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 9:38
Last modification date
20/08/2019 12:46
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