Capsule permeability via polymer and protein ingress/egress.

Details

Serval ID
serval:BIB_16546
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Capsule permeability via polymer and protein ingress/egress.
Journal
Journal of Applied Polymers Science
Author(s)
Nurdin N., Canaple L., Bartkowiak A., Desvergne B., Hunkeler D.
ISSN
0021-8995
Publication state
Published
Issued date
2000
Peer-reviewed
Oui
Volume
75
Number
9
Pages
1165-1175
Language
english
Abstract
Static incubation tests, where microcapsules and beads are contacted with polymer and protein solutions, have been developed for the characterization of permselective materials applied for bioartificial organs and drug delivery. A combination of polymer ingress, detected by size-exclusion chromatography, and protein ingress/ egress, assessed by gel electrophoresis, provides information regarding the diffusion kinetics, molar mass cutoff(MMCO) and permeability. This represents an improvement over existing permeability measurements that are based on the diffusion of a single type of solute. Specifically, the permeability of capsules based on alginate, cellulose sulfate, polymethylene-co-guanidine were characterized as a function of membrane thickness. Solid alginate beads were also evaluated. The MMCO of these capsules was estimated to be between 80 and 90 kDa using polymers, and between 116-150 kDa with proteins. Apparently, the globular shape of the proteins (radius of gyration (Rg) of 4.2-4.6 nm) facilitates their passage through the membrane, comparatively to the polysaccharide coil conformation (Rg of 6.5-8.3 nm). An increase of the capsule membrane thickness reduced these values. The MMCO of the beads, which do not have a membrane limiting their permselective properties, was higher, between 110 and 200 kDa with dextrans, and between 150 and 220 kDa with proteins. Therefore, although the permeability estimated with biologically relevant molecules is generally higher due to their lower radius of gyration, both the MMCO of synthetic and natural watersoluble polymers correlate well, and can be used as in vitro metrics for the immune protection ability of microcapsules and microbeads. This article shows, to the authors' knowledge, the first reported concordance between permeability measures based on model natural and biological macromolecules.
Keywords
Microcapsule , Ball , Control release polymer , Drug carrier , Alginates , Cellulose sulfate , Methylene copolymer , Guanidine copolymer , Molecule scattering , Permeability , Size effect , Experimental study , Biomaterial , Oside polymer , Transport properties , Microcapsule , Bille , Polymère vecteur , Vecteur médicament , Alginate , Cellulose sulfate , Méthylène copolymère , Guanidine copolymère , Diffusion molécule , Perméabilité , Effet dimensionnel , Etude expérimentale , Biomatériau , Oside polymère , Propriété transport , Microcápsula , Bola , Polímero vector , Vector medicamento , Alginato , Celulosa sulfato , Metileno copolímero , Guanidina copolímero , Difusión molécula , Permeabilidad , Efecto dimensional , Estudio experimental , Biomaterial , Osido polímero , Propiedad transporte ,
Web of science
Create date
19/11/2007 13:09
Last modification date
20/08/2019 13:45
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