Cell-Type-Specific Gene Expression Profiling in Adult Mouse Brain Reveals Normal and Disease-State Signatures.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_164A95264DF1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cell-Type-Specific Gene Expression Profiling in Adult Mouse Brain Reveals Normal and Disease-State Signatures.
Journal
Cell reports
Author(s)
Merienne N., Meunier C., Schneider A., Seguin J., Nair S.S., Rocher A.B., Le Gras S., Keime C., Faull R., Pellerin L., Chatton J.Y., Neri C., Merienne K., Déglon N.
ISSN
2211-1247 (Electronic)
Publication state
Published
Issued date
26/02/2019
Peer-reviewed
Oui
Volume
26
Number
9
Pages
2477-2493.e9
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The role of brain cell-type-specific functions and profiles in pathological and non-pathological contexts is still poorly defined. Such cell-type-specific gene expression profiles in solid, adult tissues would benefit from approaches that avoid cellular stress during isolation. Here, we developed such an approach and identified highly selective transcriptomic signatures in adult mouse striatal direct and indirect spiny projection neurons, astrocytes, and microglia. Integrating transcriptomic and epigenetic data, we obtained a comprehensive model for cell-type-specific regulation of gene expression in the mouse striatum. A cross-analysis with transcriptomic and epigenomic data generated from mouse and human Huntington's disease (HD) brains shows that opposite epigenetic mechanisms govern the transcriptional regulation of striatal neurons and glial cells and may contribute to pathogenic and compensatory mechanisms. Overall, these data validate this less stressful method for the investigation of cellular specificity in the adult mouse brain and demonstrate the potential of integrative studies using multiple databases.
Keywords
Animals, Brain/metabolism, DNA/chemistry, Epigenesis, Genetic, Gene Expression Profiling/methods, Humans, Huntington Disease/genetics, Huntington Disease/metabolism, Laser Capture Microdissection/methods, Male, Mice, Mice, Transgenic, MicroRNAs/metabolism, Nucleic Acid Conformation, RNA, Messenger/metabolism, Transcription Factors/metabolism, HD patients, HD transgenic mice, Huntington’s disease, cell-type-specific profiling, epigenetics, striatum, transcriptomics
Pubmed
Web of science
Open Access
Yes
Create date
25/03/2019 17:44
Last modification date
27/04/2020 5:20
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