High prevalence of small intestine bacteria overgrowth and asymptomatic carriage of enteric pathogens in stunted children in Antananarivo, Madagascar.

Details

Serval ID
serval:BIB_1640BF2DE312
Type
Article: article from journal or magazin.
Collection
Publications
Title
High prevalence of small intestine bacteria overgrowth and asymptomatic carriage of enteric pathogens in stunted children in Antananarivo, Madagascar.
Journal
PLoS neglected tropical diseases
Author(s)
Collard J.M., Andrianonimiadana L., Habib A., Rakotondrainipiana M., Andriantsalama P., Randriamparany R., Rabenandrasana MAN, Weill F.X., Sauvonnet N., Randremanana R.V., Guillemot V., Vonaesch P., Sansonetti P.J.
Working group(s)
Afribiota Investigators
ISSN
1935-2735 (Electronic)
ISSN-L
1935-2727
Publication state
Published
Issued date
05/2022
Peer-reviewed
Oui
Volume
16
Number
5
Pages
e0009849
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Environmental Enteric Dysfunction (EED) refers to an incompletely defined syndrome of inflammation, reduced absorptive capacity, and reduced barrier function in the small intestine. It is widespread among children and adults in low- and middle-income countries and is also associated with poor sanitation and certain gut infections possibly resulting in an abnormal gut microbiota, small intestinal bacterial overgrowth (SIBO) and stunting. We investigated bacterial pathogen exposure in stunted and non-stunted children in Antananarivo, Madagascar by collecting fecal samples from 464 children (96 severely stunted, 104 moderately stunted and 264 non-stunted) and the prevalence of SIBO in 109 duodenal aspirates from stunted children (61 from severely stunted and 48 from moderately stunted children). SIBO assessed by both aerobic and anaerobic plating techniques was very high: 85.3% when selecting a threshold of ≥105 CFU/ml of bacteria in the upper intestinal aspirates. Moreover, 58.7% of the children showed more than 106 bacteria/ml in these aspirates. The most prevalent cultivated genera recovered were Streptococcus, Neisseria, Staphylococcus, Rothia, Haemophilus, Pantoea and Branhamella. Feces screening by qPCR showed a high prevalence of bacterial enteropathogens, especially those categorized as being enteroinvasive or causing mucosal disruption, such as Shigella spp., enterotoxigenic Escherichia coli, enteropathogenic E. coli and enteroaggregative E. coli. These pathogens were detected at a similar rate in stunted children and controls, all showing no sign of severe diarrhea the day of inclusion but both living in a highly contaminated environment (slum-dwelling). Interestingly Shigella spp. was the most prevalent enteropathogen found in this study (83.3%) without overrepresentation in stunted children.
Keywords
Adult, Bacteria/genetics, Bacterial Infections, Child, Diarrhea, Escherichia coli, Feces/microbiology, Humans, Intestine, Small, Madagascar/epidemiology, Prevalence, Shigella
Pubmed
Web of science
Open Access
Yes
Create date
12/08/2022 16:30
Last modification date
07/06/2023 15:56
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