Differential expression of three T lymphocyte-activating CXC chemokines by human atheroma-associated cells

Details

Serval ID
serval:BIB_161BFF3C9AEE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential expression of three T lymphocyte-activating CXC chemokines by human atheroma-associated cells
Journal
Journal of Clinical Investigation
Author(s)
Mach  F., Sauty  A., Iarossi  A. S., Sukhova  G. K., Neote  K., Libby  P., Luster  A. D.
ISSN
0021-9738 (Print)
Publication state
Published
Issued date
10/1999
Volume
104
Number
8
Pages
1041-50
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Oct
Abstract
Activated T lymphocytes accumulate early in atheroma formation and persist at sites of lesion growth and rupture, suggesting that they may play an important role in the pathogenesis of atherosclerosis. Moreover, atherosclerotic lesions contain the Th1-type cytokine IFN-gamma, a potentiator of atherosclerosis. The present study demonstrates the differential expression of the 3 IFN-gamma-inducible CXC chemokines--IFN-inducible protein 10 (IP-10), monokine induced by IFN-gamma (Mig), and IFN-inducible T-cell alpha chemoattractant (I-TAC)--by atheroma-associated cells, as well as the expression of their receptor, CXCR3, by all T lymphocytes within human atherosclerotic lesions in situ. Atheroma-associated endothelial cells (ECs), smooth muscle cells (SMCs), and macrophages (MO) all expressed IP-10, whereas Mig and I-TAC were mainly expressed in ECs and MO, as detected by double immunofluorescence staining. ECs of microvessels within lesions also expressed abundant I-TAC. In vitro experiments supported these results and showed that IL-1beta, TNF-alpha, and CD40 ligand potentiated IP-10 expression from IFN-gamma-stimulated ECs. In addition, nitric oxide (NO) treatment decreased IFN-gamma induction of IP-10. Our findings suggest that the differential expression of IP-10, Mig, and I-TAC by atheroma-associated cells plays a role in the recruitment and retention of activated T lymphocytes observed within vascular wall lesions during atherogenesis.
Keywords
Animals Arteriosclerosis/*immunology Cells, Cultured Chemokines, CXC/*analysis/genetics Humans Immunohistochemistry *Intercellular Signaling Peptides and Proteins Nitric Oxide/physiology Penicillamine/analogs & derivatives/pharmacology RNA, Messenger/analysis Rabbits Receptors, Chemokine/analysis
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 9:52
Last modification date
20/08/2019 12:45
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