Establishment and characterization of an arsenic-sensitive monoblastic leukaemia cell line (SigM5)

Details

Serval ID
serval:BIB_15BFEB2BE806
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Establishment and characterization of an arsenic-sensitive monoblastic leukaemia cell line (SigM5)
Journal
British Journal of Haematology
Author(s)
Walter  R., Schoedon  G., Bachli  E., Betts  D. R., Hossle  J. P., Calandra  T., Joller-Jemelka  H. I., Fehr  J., Schaffner  A.
ISSN
0007-1048 (Print)
Publication state
Published
Issued date
05/2000
Volume
109
Number
2
Pages
396-404
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Few human monoblastic cell lines have been characterized to date. We have established the SigM5 cell line from a patient with acute monoblastic leukaemia (FAB M5a). Original leukaemic cells had a karyotype of 47,XY,+8, whereas the cell line showed a stemline clone of 81,XX,Y,Y,1,4,6,7,+8,+8,9,10,10,11,13,16,19[cp], with a minor sideline also present. Cytochemical staining was strongly positive with alpha-naphthylbutyrate acetate esterase, particulate positive with Sudan black and weakly positive for myeloperoxidase. Cells were positive for CD13, CD15, CD18, CD23, CD33, CD38, CD45, CD68 and myeloperoxidase. CD14 expression was 3-15%. SigM5 constitutively secreted interleukin (IL)-2, IL-8, IL-10, tumour necrosis factor (TNF)-alpha, ferritin, lysozyme, N-elastase and neopterin upon stimulation with interferon (IFN)-gamma. Cells expressed the proinflammatory mediator macrophage migration inhibitory factor (MIF). All NADPH oxidase subunits were constitutively present, but nitroblue tetrazolium reduction was only detectable upon activation with IFN-gamma. SigM5 monoblasts were sensitive to arsenic trioxide (As2O3) previously not described to induce apoptosis in monoblastic cells. Differing considerably in morphology, immunophenotype and sensitivity to arsenics from the widely used cell lines U937, HL-60 and THP-1, SigM5 is a new monoblastic cell line useful for studying leukaemogenesis, monocyte differentiation and tumour cell susceptibility to arsenic compounds.
Keywords
Antineoplastic Agents/*pharmacology Apoptosis/drug effects Arsenicals/*pharmacology Cell Culture Techniques/*methods Cell Differentiation Cell Line/immunology/pathology Chromosomes, Human, Pair 8 Humans Karyotyping Leukemia, Monocytic, Acute/genetics/*pathology Leukocytes, Mononuclear/*pathology Microscopy, Electron Oxides/*pharmacology Polyploidy
Pubmed
Web of science
Create date
25/01/2008 13:28
Last modification date
20/08/2019 12:44
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