Bilayers of nucleosome core particles

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Ressource 1Download: Leforestier2001-BiophysJ-PV.pdf (1010.11 [Ko])
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Version: Final published version
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State: Public
Version: Supplementary document
Serval ID
serval:BIB_158543397B2E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Bilayers of nucleosome core particles
Journal
Biophysical Journal
Author(s)
Leforestier  A., Dubochet  J., Livolant  F.
ISSN
0006-3495 (Print)
Publication state
Published
Issued date
10/2001
Volume
81
Number
4
Pages
2414-21
Language
english
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
Among the multiple effects involved in chromatin condensation and decondensation processes, interactions between nucleosome core particles are suspected to play a crucial role. We analyze them in the absence of linker DNA and added proteins, after the self-assembly of isolated nucleosome core particles under controlled ionic conditions. We describe an original lamellar mesophase forming tubules on the mesoscopic scale. High resolution imaging of cryosections of vitrified samples reveals how nucleosome core particles stack on top of one another into columns which themselves align to form bilayers that repel one another through a solvent layer. We deduce from this structural organization how the particles interact through attractive interactions between top and bottom faces and lateral polar interactions that originate in the heterogeneous charge distribution at the surface of the particle. These interactions, at work under conditions comparable with those found in the living cell, should be of importance in the mechanisms governing chromatin compaction in vivo.
Keywords
Animals Cattle Chickens Chromatin/chemistry/metabolism Cryoelectron Microscopy DNA/chemistry/*metabolism Histones/chemistry/*metabolism Ions/chemistry/*metabolism Macromolecular Substances Nucleosomes/chemistry/*metabolism/ultrastructure Particle Size Surface Properties
Pubmed
Open Access
Yes
Create date
24/01/2008 11:25
Last modification date
20/08/2019 13:44
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