Distinct subcellular localization of beta-tubulin epitopes in the adult mouse brain.

Details

Serval ID
serval:BIB_155EA2D0DFC1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Distinct subcellular localization of beta-tubulin epitopes in the adult mouse brain.
Journal
Histochemistry and Cell Biology
Author(s)
Nováková M., Riederer B.M., Viklický V., Dráber P.
ISSN
0948-6143 (Print)
ISSN-L
0948-6143
Publication state
Published
Issued date
1997
Volume
107
Number
4
Pages
337-344
Language
english
Abstract
A panel of monoclonal antibodies specific of alpha-tubulin (TU-01, TU-09) and beta-tubulin (TU-06, TU-13) subunits was used to study the location of N-terminal structural domains of tubulin in adult mouse brain. The specificity of antibodies was confirmed b immunoblotting experiments. Immunohistochemical staining of vibratome sections from cerebral cortex, cerebellum, hippocampus, and corpus callosum showed that antibodies TU-01, TU-09, and TU-13 reacted with neuronal and glial cells and their processes, whereas the TU-06 antibody stained only the perikarya. Dendrites and axons were either unstained or their staining was very weak. As the TU-06 epitope is located on the N-terminal structural domain of beta-tubulin, the observed staining pattern cannot be interpreted as evidence of a distinct subcellular localization of beta-tubulin isotypes or known post-translational modifications. The limited distribution of the epitope could, rather, reflect differences between the conformations of tubulin molecules in microtubules of somata and neurites or, alternatively, a specific masking of the corresponding region on the N-terminal domain of beta-tubulin by interacting protein(s) in dendrites and axons.
Keywords
Animals, Antibodies, Monoclonal, Brain Chemistry, Epitopes/analysis, Female, Mice, Subcellular Fractions/chemistry, Tubulin/analysis, Tubulin/immunology
Pubmed
Web of science
Create date
24/01/2008 14:34
Last modification date
20/08/2019 12:44
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