The antibody response in mice to carrier-free synthetic polymers of Plasmodium falciparum circumsporozoite repetitive epitope is I-Ab-restricted: possible implications for malaria vaccines.

Details

Serval ID
serval:BIB_14B517902AD9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The antibody response in mice to carrier-free synthetic polymers of Plasmodium falciparum circumsporozoite repetitive epitope is I-Ab-restricted: possible implications for malaria vaccines.
Journal
Journal of Immunology
Author(s)
Del Giudice G., Cooper J.A., Merino J., Verdini A.S., Pessi A., Togna A.R., Engers H.D., Corradin G., Lambert P.H.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Publication state
Published
Issued date
1986
Volume
137
Number
9
Pages
2952-2955
Language
english
Abstract
The immunogenicity of a novel synthetic peptide consisting of an average of 40 (Asn-Ala-Asn-Pro) repeats of the circumsporozoite protein of Plasmodium falciparum, (NANP)40, was studied in mice without using any carrier proteins. First, high titers of anti-(NANP)40 antibodies could be obtained after immunization of C57BL/6 mice. These antibodies also reacted with an extract of mosquitoes infected with P. falciparum sporozoites. C57BL/6 nu/nu mice did not produce antibodies against (NANP)40. Secondly, when 14 strains of mice with nine different H-2 haplotypes were immunized with (NANP)40 without carrier, only H-2b mice were found to produce anti-(NANP)40 antibodies, whereas all non-H-2b mice were consistently unresponsive. This response was demonstrated to be I-A-linked by using recombinant and mutant mice. I-Ab [B10.A(5R)] mice produced anti-(NANP)40 antibodies as well as H-2b inbred mice. B6CH-2bm12 I-Ab-mutant mice showed only a very low response. Third, the antibody response against (NANP)40 could be induced in nonresponder mice by immunization with the peptide coupled to a carrier protein. In view of the existence of such an exceptional H-2b restriction in the response to sporozoite synthetic peptides in mice, the triggering of peptide-specific T cell responses in humans receiving sporozoite malaria vaccines might be difficult to achieve.
Keywords
Animals, Antibody Formation, Antigens/immunology, Antigens, Protozoan/immunology, Carrier Proteins/immunology, Histocompatibility Antigens Class II/immunology, Mice, Mice, Inbred Strains, Peptides/chemical synthesis, Peptides/immunology, Plasmodium falciparum/immunology, Vaccines, Synthetic/immunology
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 12:43
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