Beta-adrenergic modulation of lung fluid balance in acute P aeruginosa pneumonia in rats

Details

Serval ID
serval:BIB_148D3674AA8C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Beta-adrenergic modulation of lung fluid balance in acute P aeruginosa pneumonia in rats
Journal
Exp Lung Res
Author(s)
Robriquet L., Kipnis E., Guery B.
ISSN
1521-0499 (Electronic)
ISSN-L
0190-2148
Publication state
Published
Issued date
10/2011
Volume
37
Number
8
Pages
453-60
Language
english
Notes
Robriquet, Laurent
Kipnis, Eric
Guery, Benoit
eng
England
Exp Lung Res. 2011 Oct;37(8):453-60. doi: 10.3109/01902148.2011.593092. Epub 2011 Sep 6.
Abstract
OBJECTIVE: we investigated the effects of terbutaline, a ss(2)-adrenergic agonist, on lung permeability and alveolar fluid clearance (AFC) in acute lung injury (ALI). METHODS: the study was conducted in vivo on a rat model of P aeruginosa (Pa)-induced ALI. Rats were randomly divided into five groups: the control group (saline group), Pa and saline group, Pa and terbutaline treated group receiving intratracheal instillation of terbutaline at 10(-4) M, Pa and terbutaline plus propranolol treated group (terbutaline+propranolol group) and Pa and propranolol treated group (propranolol group). Hemodynamics, airway pressures, arterial blood gases, extravascular lung water, lung permeability to protein evaluated by the extravascular accumulation of (125)I-albumin (EPE), bacterial counts, and alveolar fluid clearance (AFC) were measured. RESULTS: 4.5 hours after bacterial instillation, the lung wet-to-dry ratio and the EPE were significantly decreased in the terbutaline group compared to saline control group (respectively 4.31 +/- 0.51 g/g versus 5.99 +/- 0.5 g/g 4.18 +/- 0.25 g/g and 148 +/- 68 muL versus 349 +/- 97 muL respectively p < 0.01). Treatment with terbutaline in the Pa-instilled group significantly increased basal AFC compared with the saline and Pa group, (respectively 22.3 +/- 1.3% versus 12.5 +/- 4.7%, p < 0.001). Intratracheal instillation of propranolol (10(-4) M) inhibited the effects of terbutaline on lung fluid balance. CONCLUSION: Exogenous instillation of beta2-adrenergic have a beneficial effect on lung fluid balance following Pa pneumonia in rats, by reducing pulmonary endothelial permeability and increasing alveolar fluid clearance. These data suggest that exogenous beta-adrenergic therapy can protect against alveolar edema formation in acute P aeruginosa pneumonia.
Keywords
Acute Disease, Acute Lung Injury, Adrenergic beta-2 Receptor Agonists/*pharmacology, Animals, Capillary Permeability/drug effects, Lung/blood supply/drug effects/physiopathology, Male, Pneumonia, Bacterial/*drug therapy/*physiopathology, Pseudomonas Infections/*drug therapy/*physiopathology, Pulmonary Edema/prevention & control, Rats, Rats, Sprague-Dawley, Terbutaline/*pharmacology, Water-Electrolyte Balance/*drug effects
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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