Altered expression of CD44 and DKK1 in the progression of Barrett's esophagus to esophageal adenocarcinoma.

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Serval ID
serval:BIB_147B56E95722
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Altered expression of CD44 and DKK1 in the progression of Barrett's esophagus to esophageal adenocarcinoma.
Journal
Virchows Archiv
Author(s)
Darlavoix T., Seelentag W., Yan P., Bachmann A., Bosman F.T.
ISSN
1432-2307 (Electronic)
ISSN-L
0945-6317
Publication state
Published
Issued date
2009
Volume
454
Number
6
Pages
629-637
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Barrett's esophagus (BE) is an acquired condition in which the normal lining of the esophagus is replaced by intestinal metaplastic epithelium. BE can evolve to esophageal adenocarcinoma (EAC) through low-grade dysplasia (LGD) and high-grade dysplasia (HGD). The only generally accepted marker for increased risk of EAC is the presence of HGD, diagnosed on endoscopic biopsies. More specific markers for the prediction of EAC risk are needed. A tissue microarray was constructed comprising tissue samples from BE, LGD, HGD, and EAC. Marker expression was studied by immunohistochemistry using antibodies against CD44, DKK1, CDX2, COX2, SOX9, OCT1, E-cadherin, and beta-catenin. Immunostaining was evaluated semi-quantitatively. CD44 expression decreased in HGD and EAC relative to BE and LGD. DKK1 expression increased in HGD and EAC relative to BE and LDG. CDX2 expression increased in HGD but decreased in EAC. COX2 expression decreased in EAC, and SOX9 expression increased only in the upper crypt epithelial cells in HGD. E-cadherin expression decreased in EAC. Nuclear beta-catenin was not significantly different between BE, LGD, and HGD. Loss of CD44 and gain of DKK1 expression characterizes progression from BE and LGD to HGD and EAC, and their altered expression might indicate an increased risk for developing an EAC. This observation warrants inclusion of these immunohistochemically detectable markers in a study with a long patient follow-up.
Keywords
Adenocarcinoma/diagnosis, Adenocarcinoma/metabolism, Antigens, CD44/metabolism, Barrett Esophagus/diagnosis, Barrett Esophagus/metabolism, Cyclooxygenase 2/metabolism, Disease Progression, Esophageal Neoplasms/diagnosis, Esophageal Neoplasms/metabolism, Fluorescent Antibody Technique, Direct, Homeodomain Proteins/metabolism, Humans, Immunoenzyme Techniques, Intercellular Signaling Peptides and Proteins/metabolism, Precancerous Conditions/metabolism, Precancerous Conditions/pathology, Tissue Array Analysis, Tumor Markers, Biological/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
18/05/2009 18:26
Last modification date
01/10/2019 7:16
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