Rapid Liquid Chromatography-Tandem Mass Spectrometry Analysis of Two Urinary Oxidative Stress Biomarkers: 8-oxodG and 8-isoprostane.

Details

Serval ID
serval:BIB_14714E7AD9F5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rapid Liquid Chromatography-Tandem Mass Spectrometry Analysis of Two Urinary Oxidative Stress Biomarkers: 8-oxodG and 8-isoprostane.
Journal
Antioxidants
Author(s)
Sambiagio N., Sauvain J.J., Berthet A., Auer R., Schoeni A., Hopf N.B.
ISSN
2076-3921 (Print)
ISSN-L
2076-3921
Publication state
Published
Issued date
31/12/2020
Peer-reviewed
Oui
Volume
10
Number
1
Pages
E38
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Human biomonitoring of oxidative stress relies on urinary effect biomarkers such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), and 8-iso-prostaglandin F2α (8-isoprostane); however, their levels reported for similar populations are inconsistent in the scientific literature. One of the reasons is the multitude of analytical methods with varying degrees of selectivity used to quantify these biomarkers. Single-analyte methods are often used, requiring multiple injections that increase both time and cost. We developed a rapid ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to quantify both urinary biomarkers simultaneously. A reversed-phase column using a gradient consisting of 0.1% acetic acid in water and 0.1% acetic acid in methanol/acetonitrile (70:30) was used for separation. The MS detection was by positive (8-oxodG) and negative (8-isoprostane) ion-mode by multiple reaction monitoring. Very low limit of detection (<20 pg/mL), excellent linearity (R2 > 0.999), accuracy (near 100%), and precision (CV < 10%) both for intra-day and inter-day experiments were achieved, as well as high recovery rates (>91%). Matrix effects were observed but were compensated by using internal standards. Our newly developed method is applicable for biomonitoring studies as well as large epidemiological studies investigating the effect of oxidative damage, as it requires only minimal clean up using solid phase extraction.
Keywords
8-isoprostane, 8-oxodG, biomarker, biomonitoring, liquid chromatography, mass spectrometry, oxidative stress
Pubmed
Open Access
Yes
Create date
11/01/2021 14:24
Last modification date
19/01/2021 7:26
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