A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii and Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_14611B9C4DE7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii and Pseudomonas aeruginosa.
Journal
Journal of clinical microbiology
Author(s)
Lescat M., Poirel L., Tinguely C., Nordmann P.
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Publication state
Published
Issued date
03/2019
Peer-reviewed
Oui
Volume
57
Number
3
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
A rapid test was developed for identification of polymyxin resistance in nonfermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, as observed by its color change (blue to purple or pink). Its evaluation was performed by using 92 colistin-resistant and colistin-susceptible Acinetobacter baumannii and Pseudomonas aeruginosa isolates. Sensitivity and specificity were found to be 100% and 95%, respectively, by comparison with the standard broth microdilution method. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is inexpensive, easy to perform, highly sensitive and specific, and can be completed in 4 hours. It could be useful in countries facing endemic spread of colistin-resistant nonfermenters.
Keywords
Acinetobacter baumannii/drug effects, Anti-Bacterial Agents/pharmacology, Drug Resistance, Bacterial, Humans, Microbial Sensitivity Tests/economics, Microbial Sensitivity Tests/methods, Oxazines, Polymyxins/pharmacology, Pseudomonas aeruginosa/drug effects, Sensitivity and Specificity, Time Factors, Xanthenes, Acinetobacter baumannii, Pseudomonas aeruginosa, colistin, rapid diagnostic test, susceptibility testing
Pubmed
Web of science
Open Access
Yes
Create date
17/03/2019 16:27
Last modification date
26/06/2020 5:21
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