Spatial expression patterns of epidermal growth factor receptor gene transcripts in the postnatal mammalian cochlea.

Details

Serval ID
serval:BIB_14101
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatial expression patterns of epidermal growth factor receptor gene transcripts in the postnatal mammalian cochlea.
Journal
Hearing research
Author(s)
Zine A., Nyffeler M., de Ribaupierre F.
ISSN
0378-5955 (Print)
ISSN-L
0378-5955
Publication state
Published
Issued date
03/2000
Peer-reviewed
Oui
Volume
141
Number
1-2
Pages
19-27
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Recent in vitro studies demonstrated that members of the epidermal growth factor (EGF) family are involved in hair cell replacement in the postnatal mammalian organ of Corti (OC) after ototoxic damage. This suggests a role for the EGF receptor (EGFR) in this process. We examined the expression of EGFR mRNA within the normal postnatal day 3 (P3) and adult rat cochlear epithelium by RT-PCR and examined its cellular localization with non-radioactive in situ hybridization in P3 and adult cochleae. RT-PCR demonstrated that EGFR mRNA is expressed in P3 and adult cochlear epithelium. In situ hybridization localized high levels of EGFR transcripts in the OC, spiral ganglion, Kölliker's organ and detectable levels in the supporting cells and the stria vascularis of P3 cochlea. In the adult cochlea, EGFR transcripts were detected only in the spiral ganglion. Our results support that the EGFR is implicated in the differentiation of several cochlear cell types and in the response of OC to ototoxic damage of the P3 rat. In the adult, it may participate in the maintenance of the mature neurons and its absence in the OC may contribute to the lack of regenerative responses in the adult cochlea.
Keywords
Animals, Animals, Newborn, Base Sequence, Cochlea/growth & development, Cochlea/injuries, Cochlea/metabolism, DNA Primers/genetics, Epithelium/metabolism, ErbB Receptors/genetics, Gene Expression Regulation, Developmental, In Situ Hybridization, Organ of Corti/growth & development, Organ of Corti/injuries, Organ of Corti/metabolism, RNA, Messenger/genetics, RNA, Messenger/metabolism, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Create date
19/11/2007 12:06
Last modification date
09/05/2023 5:53
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