New strategy for carbon monoxide poisoning diagnosis: Carboxyhemoglobin (COHb) vs Total Blood Carbon Monoxide (TBCO).

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State: Public
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_140266EE9521
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
New strategy for carbon monoxide poisoning diagnosis: Carboxyhemoglobin (COHb) vs Total Blood Carbon Monoxide (TBCO).
Journal
Forensic science international
Author(s)
Oliverio S., Varlet V.
ISSN
1872-6283 (Electronic)
ISSN-L
0379-0738
Publication state
Published
Issued date
01/2020
Peer-reviewed
Oui
Volume
306
Pages
110063
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Abstract
Diagnosis of carbon monoxide (CO) poisonings has always been a challenging task due to the susceptibility to alterations of the optical state and degradation of blood samples during sampling, transport and storage, which highly affects the analysis with spectrophotometric methods. Methodological improvements are then required urgently because of increased reports of cases with discrepancies between results of the measured biomarker carboxyhemoglobin (COHb) and reported symptoms. Total blood CO (TBCO) measured chromatographically was thus proposed in a previous study as alternative biomarker to COHb. This approach was investigated in this study by comparing the two biomarkers and assessing the effects of various storage parameters (temperature, preservative, time, tube headspace (HS) volume, initial saturation level, freeze- and thaw- and reopening-cycles) over a period of one month. Results show that while for TBCO, concentrations are relatively stable over the observation period regardless of parameters such as temperature, time and HS volume, for COHb, concentrations are altered significantly during storage. Therefore, the use of TBCO as alternative biomarker for CO poisonings has been proposed, since it provides more valid results and is more stable even under non-optimal storage conditions. Additionally, it can be used to predict COHb in cases where sample degradation hinders optical measurement. Furthermore, a correction formula for COHb and TBCO is provided to be used in laboratories or circumstances where optimal storage or analysis is not possible, to obtain more accurate results.
Keywords
Biomarkers/blood, Carbon Monoxide/blood, Carbon Monoxide Poisoning/diagnosis, Carboxyhemoglobin/analysis, Chromatography, Gas, Data Interpretation, Statistical, Forensic Medicine/methods, Humans, Oximetry, Specimen Handling, Spectrophotometry, Blood analysis, COHb, Carbon monoxide poisoning, Carboxyhemoglobin, GC–MS, Storage conditions, TBCO
Pubmed
Web of science
Open Access
Yes
Create date
04/12/2019 23:03
Last modification date
11/02/2020 7:19
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