Systematic Investigation of SARS-CoV-2 Receptor Protein Distribution along Viral Entry Routes in Humans.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_13F142E3F61A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Systematic Investigation of SARS-CoV-2 Receptor Protein Distribution along Viral Entry Routes in Humans.
Journal
Respiration; international review of thoracic diseases
Author(s)
Bräutigam K., Reinhard S., Galván J.A., Wartenberg M., Hewer E., Schürch C.M.
ISSN
1423-0356 (Electronic)
ISSN-L
0025-7931
Publication state
Published
Issued date
2022
Peer-reviewed
Oui
Volume
101
Number
6
Pages
610-618
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The novel beta-coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), enters the human body via mucosal surfaces of the upper and/or lower respiratory tract. Viral entry into epithelial cells is mediated via angiotensin-converting enzyme 2 (ACE2) and auxiliary molecules, but the precise anatomic site of infection still remains unclear.
Here, we systematically investigated the main SARS-CoV-2 receptor proteins ACE2 and transmembrane serine protease 2 (TMPRSS2), as well as 2 molecules potentially involved in viral entry, furin and CD147, in formalin-fixed, paraffin-embedded human tissues. Tissue microarrays incorporating a total of 879 tissue cores from conjunctival (n = 84), sinonasal (n = 95), and lung (bronchiolar/alveolar; n = 96) specimens were investigated for protein expression by immunohistochemistry.
ACE2 and TMPRSS2 were expressed in ciliated epithelial cells of the conjunctivae and sinonasal tissues, with highest expression levels observed in the apical cilia. In contrast, in the lung, the expression of those molecules in bronchiolar and alveolar epithelial cells was much rarer and only very focal when present. Furin and CD147 were more uniformly expressed in all tissues analyzed, including the lung. Interestingly, alveolar macrophages consistently expressed high levels of all 4 molecules investigated.
Our study confirms and extends previous findings and contributes to a better understanding of potential SARS-CoV-2 infection sites along the human respiratory tract.
Keywords
Angiotensin-converting enzyme 2, CD147, Coronavirus disease-19, Furin, Immunohistochemistry, Severe acute respiratory syndrome coronavirus 2, Tissue microarray, Transmembrane serine protease 2
Pubmed
Web of science
Open Access
Yes
Create date
20/01/2022 9:49
Last modification date
22/07/2022 6:08
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