Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture.

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Serval ID
serval:BIB_138F8EED0F06
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture.
Journal
Histochemistry and Cell Biology
Author(s)
Bouchet-Marquis C., Dubochet J., Fakan S.
ISSN
0948-6143
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
125
Number
1-2
Pages
43-51
Language
english
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Jan
Abstract
Cryoelectron microscopy of vitrified sections has become a powerful tool for investigating the fine structural features of cellular compartments. In the present study, this approach has been applied in order to explore the ultrastructural morphology of the interphase nucleus in different mammalian cultured cells. Rat hepatoma, Chinese hamster ovary and Potorus kidney cells were cryofixed by high-pressure freezing and the cryosections were examined at low temperature by transmission electron microscopy. Our results show that while the contrast of nuclear structural domains is remarkably homogeneous in hydrated sections, some of them can be recognised due to their characteristic texture. Thus, condensed chromatin appears finely granular and the perichromatin region contains rather abundant fibro-granular elements suggesting the presence of dispersed chromatin fibres and of perichromatin fibrils and granules. The interchromatin space looks homogeneous and interchromatin granules have not been identified under these preparative conditions. In the nucleolus, the most striking feature is the granular component, while the other parts of the nucleolar body, which appear less contrasted, are difficult to resolve. The nuclear envelope is easily recognisable with its regular perinuclear space and nuclear pore complexes. Our observations are discussed in the context of results obtained by other, more conventional electron microscopic methods.
Keywords
Animals, CHO Cells, Cell Nucleus, Chromatin, Cricetinae, Cryoelectron Microscopy, Cryopreservation, Freezing, Lipid Bilayers, Rats, Tissue Fixation
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 11:25
Last modification date
01/10/2019 7:16
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