Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles.

Details

Serval ID
serval:BIB_12CB24FE119D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles.
Journal
Cell reports
Author(s)
Cianciaruso C., Beltraminelli T., Duval F., Nassiri S., Hamelin R., Mozes A., Gallart-Ayala H., Ceada Torres G., Torchia B., Ries C.H., Ivanisevic J., De Palma M.
ISSN
2211-1247 (Electronic)
Publication state
Published
Issued date
04/06/2019
Peer-reviewed
Oui
Volume
27
Number
10
Pages
3062-3080.e11
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Extracellular vesicles (EVs), including exosomes, modulate multiple aspects of cancer biology. Tumor-associated macrophages (TAMs) secrete EVs, but their molecular features and functions are poorly characterized. Here, we report methodology for the enrichment, quantification, and proteomic and lipidomic analysis of EVs released from mouse TAMs (TAM-EVs). Compared to source TAMs, TAM-EVs present molecular profiles associated with a Th1/M1 polarization signature, enhanced inflammation and immune response, and a more favorable patient prognosis. Accordingly, enriched TAM-EV preparations promote T cell proliferation and activation ex vivo. TAM-EVs also contain bioactive lipids and biosynthetic enzymes, which may alter pro-inflammatory signaling in the cancer cells. Thus, whereas TAMs are largely immunosuppressive, their EVs may have the potential to stimulate, rather than limit, anti-tumor immunity.
Keywords
Animals, Antibodies/therapeutic use, Bone Marrow Cells/cytology, Cell Line, Tumor, Cell Proliferation, Colorectal Neoplasms/drug therapy, Colorectal Neoplasms/metabolism, Colorectal Neoplasms/pathology, Extracellular Vesicles/metabolism, Female, Interleukin-4/pharmacology, Lipopolysaccharides/pharmacology, Macrophages/cytology, Macrophages/drug effects, Macrophages/metabolism, Male, Mice, Mice, Inbred C57BL, Protein Interaction Maps, Proteome/analysis, Proteomics, Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/immunology, Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/metabolism, Th1 Cells/cytology, Th1 Cells/immunology, Th1 Cells/metabolism, Transplantation, Homologous, T cell response, exosome, extracellular vesicle, inflammation, lipid metabolism, lipidomics, proteomics, tumor microenvironment, tumor-associated macrophage
Pubmed
Web of science
Open Access
Yes
Create date
01/07/2019 16:16
Last modification date
26/08/2020 5:22
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