Role of the stress sigma factor RpoS in GacA/RsmA-controlled secondary metabolism and resistance to oxidative stress in Pseudomonas fluorescens CHA0.

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Serval ID
serval:BIB_128308AA67F4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Role of the stress sigma factor RpoS in GacA/RsmA-controlled secondary metabolism and resistance to oxidative stress in Pseudomonas fluorescens CHA0.
Journal
FEMS Microbiology Letters
Author(s)
Heeb S., Valverde C., Gigot-Bonnefoy C., Haas D.
ISSN
0378-1097[print], 0378-1097[linking]
Publication state
Published
Issued date
2005
Volume
243
Number
1
Pages
251-258
Language
english
Abstract
In Pseudomonas fluorescens biocontrol strain CHA0, the two-component system GacS/GacA positively controls the synthesis of extracellular products such as hydrogen cyanide, protease, and 2,4-diacetylphloroglucinol, by upregulating the transcription of small regulatory RNAs which relieve RsmA-mediated translational repression of target genes. The expression of the stress sigma factor sigmaS (RpoS) was controlled positively by GacA and negatively by RsmA. By comparison with the wild-type CHA0, both a gacS and an rpoS null mutant were more sensitive to H2O2 in stationary phase. Overexpression of rpoS or of rsmZ, encoding a small RNA antagonistic to RsmA, restored peroxide resistance to a gacS mutant. By contrast, the rpoS mutant showed a slight increase in the expression of the hcnA (HCN synthase subunit) gene and of the aprA (major exoprotease) gene, whereas overexpression of sigmaS strongly reduced the expression of these genes. These results suggest that in strain CHA0, regulation of exoproduct synthesis does not involve sigmaS as an intermediate in the Gac/Rsm signal transduction pathway whereas sigmaS participates in Gac/Rsm-mediated resistance to oxidative stress.
Keywords
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Exopeptidases/genetics, Exopeptidases/metabolism, Gene Expression Regulation, Bacterial, Hydrogen Peroxide/pharmacology, Multienzyme Complexes/genetics, Multienzyme Complexes/metabolism, Oxidative Stress, Oxidoreductases/genetics, Oxidoreductases/metabolism, Oxidoreductases Acting on CH-NH2 Group Donors, Pseudomonas fluorescens/genetics, Pseudomonas fluorescens/growth & development, RNA-Binding Proteins/genetics, RNA-Binding Proteins/metabolism, Repressor Proteins/genetics, Repressor Proteins/metabolism, Sigma Factor/genetics, Sigma Factor/metabolism, Signal Transduction
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 17:01
Last modification date
25/09/2019 6:08
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