Different Effects of BORIS/CTCFL on Stemness Gene Expression, Sphere Formation and Cell Survival in Epithelial Cancer Stem Cells.

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State: Public
Version: Final published version
Serval ID
serval:BIB_127E6A07B81B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Different Effects of BORIS/CTCFL on Stemness Gene Expression, Sphere Formation and Cell Survival in Epithelial Cancer Stem Cells.
Journal
Plos One
Author(s)
Alberti L., Losi L., Leyvraz S., Benhattar J.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
10
Number
7
Pages
e0132977
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Abstract
Cancer stem cells are cancer cells characterized by stem cell properties and represent a small population of tumor cells that drives tumor development, progression, metastasis and drug resistance. To date, the molecular mechanisms that generate and regulate cancer stem cells are not well defined. BORIS (Brother of Regulator of Imprinted Sites) or CTCFL (CTCF-like) is a DNA-binding protein that is expressed in normal tissues only in germ cells and is re-activated in tumors. Recent evidences have highlighted the correlation of BORIS/CTCFL expression with poor overall survival of different cancer patients. We have previously shown an association of BORIS-expressing cells with stemness gene expression in embryonic cancer cells. Here, we studied the role of BORIS in epithelial tumor cells. Using BORIS-molecular beacon that was already validated, we were able to show the presence of BORIS mRNA in cancer stem cell-enriched populations (side population and spheres) of cervical, colon and breast tumor cells. BORIS silencing studies showed a decrease of sphere formation capacity in breast and colon tumor cells. Importantly, BORIS-silencing led to down-regulation of hTERT, stem cell (NANOG, OCT4, SOX2 and BMI1) and cancer stem cell markers (ABCG2, CD44 and ALDH1) genes. Conversely, BORIS-induction led to up-regulation of the same genes. These phenotypes were observed in cervical, colon and invasive breast tumor cells. However, a completely different behavior was observed in the non-invasive breast tumor cells (MCF7). Indeed, these cells acquired an epithelial mesenchymal transition phenotype after BORIS silencing. Our results demonstrate that BORIS is associated with cancer stem cell-enriched populations of several epithelial tumor cells and the different phenotypes depend on the origin of tumor cells.
Keywords
ATP-Binding Cassette Transporters/genetics, ATP-Binding Cassette Transporters/metabolism, Antigens, CD44/genetics, Antigens, CD44/metabolism, Biomarkers, Tumor/genetics, Biomarkers, Tumor/metabolism, Cell Line, Tumor, Cell Movement, Cell Proliferation, DNA-Binding Proteins/antagonists & inhibitors, DNA-Binding Proteins/genetics, Epithelial Cells/metabolism, Epithelial Cells/pathology, Epithelial-Mesenchymal Transition/genetics, Gene Expression Regulation, Neoplastic, Homeodomain Proteins/genetics, Homeodomain Proteins/metabolism, Humans, Isoenzymes/genetics, Isoenzymes/metabolism, Neoplasm Proteins/genetics, Neoplasm Proteins/metabolism, Neoplastic Stem Cells/metabolism, Neoplastic Stem Cells/pathology, Octamer Transcription Factor-3/genetics, Octamer Transcription Factor-3/metabolism, Organ Specificity, Phenotype, Polycomb Repressive Complex 1/genetics, Polycomb Repressive Complex 1/metabolism, RNA, Small Interfering/genetics, RNA, Small Interfering/metabolism, Retinal Dehydrogenase/genetics, Retinal Dehydrogenase/metabolism, SOXB1 Transcription Factors/genetics, SOXB1 Transcription Factors/metabolism, Signal Transduction, Spheroids, Cellular/metabolism, Spheroids, Cellular/pathology, Telomerase/genetics, Telomerase/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/08/2015 16:41
Last modification date
20/08/2019 12:40
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