Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain.

Details

Serval ID
serval:BIB_12782
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain.
Journal
Neuroscience letters
Author(s)
Braissant O., Honegger P., Loup M., Iwase K., Takiguchi M., Bachmann C.
ISSN
0304-3940 (Print)
ISSN-L
0304-3940
Publication state
Published
Issued date
07/05/1999
Peer-reviewed
Oui
Volume
266
Number
2
Pages
89-92
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Hyperammonemia in the brain leads to poorly understood alterations of nitric oxide (NO) synthesis. Arginine, the substrate of nitric oxide synthases, might be recycled from the citrulline produced with NO by argininosuccinate synthetase (AS) and argininosuccinate lyase (AL). The regulation of AS and AL genes during hyperammonemia is unknown in the brain. We used brain cell aggregates cultured from dissociated telencephalic cortex of rat embryos to analyze the regulation of AS and AL genes in hyperammonemia. Using RNase protection assay and non-radioactive in situ hybridization on aggregate cryosections, we show that both AS and AL genes are induced in astrocytes but not in neurons of aggregates exposed to 5 mM NH4Cl. Our work suggests that the hyperammonemic brain might increase its recycling of citrulline to arginine.
Keywords
Ammonia/blood, Animals, Argininosuccinate Lyase/genetics, Argininosuccinate Synthase/genetics, Brain/cytology, Brain/embryology, Brain/metabolism, Cell Aggregation/physiology, Cells, Cultured, Embryonic and Fetal Development/physiology, Gene Expression Regulation, Developmental/physiology, Gene Expression Regulation, Enzymologic/physiology, Rats
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:04
Last modification date
10/09/2019 5:26
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