Modulation of proteolytic activity in tissues following chronic inhibition of angiotensin-converting enzyme

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_12186820C0C0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Modulation of proteolytic activity in tissues following chronic inhibition of angiotensin-converting enzyme
Journal
Biochemical Pharmacology
Author(s)
Juillerat-Jeanneret L.
ISSN
0006-2952 (Print)
Publication state
Published
Issued date
1993
Volume
45
Number
7
Pages
1447-1454
Language
english
Notes
PT - Journal Article
Abstract
The release of angiotensin-converting enzyme (ACE) (EC 3.4.15.1) from aortic rings and the modulation of the proteolytic balance of rat organs under chronic ACE inhibition were examined. ACE from rat organs had a higher apparent molecular mass than the circulating enzyme, but a similar behavior towards ACE inhibitors. Chronic treatment with ACE inhibitors (captopril or lisinopril) for 25 days, followed by 1 day without treatment, increased plasma ACE, but only slightly modified lung, aorta, heart and kidney specific ACE activity. In the lung the activities of aminopeptidases A and B, two angiotensin degrading enzymes, decreased, as did the activity of aminopeptidase A in the plasma. In vitro, the release of ACE from aortic rings was not suppressed by inhibitors of either serine proteases, metalloproteases, serine and thiol proteases, or aspartyl proteases. After chronic ACE inhibition, the release of ACE from aortic rings was not significantly modified by the presence of protease inhibitors. As shown by gel filtration experiments, ACE was converted from its tissue form into its circulating form only after release from the endothelium
Keywords
Aminopeptidases/analysis/blood/Angiotensin-Converting Enzyme Inhibitors/pharmacology/Animals/Aorta/enzymology/Brain/Carboxypeptidases/Cells,Cultured/Kidney/Myocardium/Peptidyl-Dipeptidase A/Proteins/metabolism/Rats/gamma-Glutamyltransferase
Pubmed
Web of science
Create date
29/01/2008 18:33
Last modification date
11/06/2020 6:08
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