Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.

Details

Ressource 1Request a copy Sous embargo indéterminé.
State: Public
Version: author
Serval ID
serval:BIB_119B3D9C5371
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.
Journal
Neuroscience Letters
Author(s)
Noelker C., Schwake M., Balzer-Geldsetzer M., Bacher M., Popp J., Schlegel J., Eggert K., Oertel W.H., Klockgether T., Dodel R.C.
ISSN
1872-7972 (Electronic)
ISSN-L
0304-3940
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
507
Number
1
Pages
10-15
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by progressive loss of dopaminergic (DA) neurons of the substantia nigra pars compacta with unknown aetiology. 6-Hydroxydopamine (6-OHDA) treatment of neuronal cells is an established in vivo model for mimicking the effect of oxidative stress found in PD brains. We examined the effects of 6-OHDA treatment on human neuroblastoma cells (SH-SY5Y) and primary mesencephalic cultures. Using a reverse arbitrarily primed polymerase chain reaction (RAP-PCR) approach we generated reproducible genetic fingerprints of differential expression levels in cell cultures treated with 6-OHDA. Of the resulting sequences, 23 showed considerable homology to known human coding sequences. The results of the RAP-PCR were validated by reverse transcription PCR, real-time PCR and, for selected genes, by Western blot analysis and immunofluorescence. In four cases, [tomoregulin-1 (TMEFF-1), collapsin response mediator protein 1 (CRMP-1), neurexin-1, and phosphoribosylaminoimidazole synthetase (GART)], a down-regulation of mRNA and protein levels was detected. Further studies will be necessary on the physiological role of the identified proteins and their impact on pathways leading to neurodegeneration in PD.
Pubmed
Web of science
Create date
20/01/2012 12:27
Last modification date
26/01/2020 8:08
Usage data