Normal hemopoiesis and lymphopoiesis in the combined absence of numb and numblike.

Details

Serval ID
serval:BIB_10FDF4617FC8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Normal hemopoiesis and lymphopoiesis in the combined absence of numb and numblike.
Journal
Journal of immunology
Author(s)
Wilson A., Ardiet D.L., Saner C., Vilain N., Beermann F., Aguet M., Macdonald H.R., Zilian O.
ISSN
0022-1767
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
178
Number
11
Pages
6746-6751
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The mammalian ortholog of the conserved Drosophila adaptor protein Numb (Nb) and its homolog Numblike (Nbl) modulate neuronal cell fate determination at least in part by antagonizing Notch signaling. Because the Notch pathway has been implicated in regulating hemopoietic stem cell self-renewal and T cell fate specification in mammals, we investigated the role of Nb and Nbl in hemopoiesis using conditional gene targeting. Surprisingly simultaneous deletion of both Nb and Nbl in murine bone marrow precursors did not affect the ability of stem cells to self-renew or to give rise to differentiated myeloid or lymphoid progeny, even under competitive conditions in mixed chimeras. Furthermore, T cell fate specification and intrathymic T cell development were unaffected in the combined absence of Nb and Nbl. Collectively our data indicate that the Nb family of adaptor proteins is dispensable for hemopoiesis and lymphopoiesis in mice, despite their proposed role in neuronal stem cell development.
Keywords
Animals, Bone Marrow Cells/cytology, Bone Marrow Cells/metabolism, Cell Differentiation/genetics, Cell Differentiation/immunology, Cell Lineage/genetics, Cell Lineage/immunology, Hematopoiesis/genetics, Hematopoiesis/immunology, Hematopoietic Stem Cells/cytology, Hematopoietic Stem Cells/metabolism, Lymphopoiesis/genetics, Lymphopoiesis/immunology, Membrane Proteins/biosynthesis, Membrane Proteins/deficiency, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Nerve Tissue Proteins/biosynthesis, Nerve Tissue Proteins/deficiency, Neurons/cytology, Neurons/metabolism, Thymus Gland/cytology, Thymus Gland/metabolism
Pubmed
Web of science
Create date
28/01/2008 11:36
Last modification date
20/08/2019 12:38
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