Connective tissue growth factor, steatosis and fibrosis in patients with chronic hepatitis C.

Details

Serval ID
serval:BIB_10A45328AFF6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Connective tissue growth factor, steatosis and fibrosis in patients with chronic hepatitis C.
Journal
Liver International
Author(s)
Hora C., Negro F., Leandro G., Oneta C.M., Rubbia-Brandt L., Muellhaupt B., Helbling B., Malinverni R., Gonvers J.J., Dufour J.F.
ISSN
1478-3223
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
28
Number
3
Pages
370-376
Language
english
Abstract
BACKGROUND/AIM: Both steatosis and insulin resistance have been linked to accelerated fibrosis in chronic hepatitis C. Connective tissue growth factor (CTGF) plays a major role in extracellular matrix production in fibrotic disorders including cirrhosis, and its expression is stimulated in vitro by insulin and glucose. We hypothesized that CTGF may link steatosis, insulin resistance and fibrosis. METHODS: We included 153 chronic hepatitis C patients enrolled in the Swiss Hepatitis C Cohort Study and for whom a liver biopsy and plasma samples were available. CTGF expression was assessed quantitatively by immunohistochemistry. In 94 patients (57 with genotypes non-3), plasma levels of glucose, insulin and leptin were also measured. CTGF synthesis was investigated by immunoblotting on LX-2 stellate cells. RESULTS: Connective tissue growth factor expression was higher in patients with steatosis (P=0.039) and in patients with fibrosis (P=0.008) than those without these features. CTGF levels were neither associated with insulinaemia or with glycaemia, nor with inflammation. By multiple regression analysis, CTGF levels were independently associated with steatosis, a past history of alcohol abuse, plasma leptin and HCV RNA levels; when only patients with genotypes non-3 were considered, CTGF levels were independently associated with a past history of alcohol abuse, plasma leptin levels and steatosis. Leptin stimulated CTGF synthesis in LX-2 cells. CONCLUSIONS: In patients with chronic hepatitis C and steatosis, CTGF may promote fibrosis independently of inflammation. CTGF may link steatosis and fibrosis via increased leptin levels.
Keywords
Adult, Blood Glucose/analysis, Connective Tissue Growth Factor, Fatty Liver/etiology, Female, Hepatitis C, Chronic/complications, Humans, Immediate-Early Proteins/metabolism, Immunoblotting, Immunohistochemistry, Insulin/blood, Intercellular Signaling Peptides and Proteins/metabolism, Leptin/blood, Liver Cirrhosis/etiology, Male, Middle Aged, Switzerland
Pubmed
Web of science
Create date
13/10/2009 10:42
Last modification date
20/08/2019 12:37
Usage data