Information transfer between large and small two-dimensional polyacrylamide gel electrophoresis.

Details

Serval ID
serval:BIB_10816
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Information transfer between large and small two-dimensional polyacrylamide gel electrophoresis.
Journal
Electrophoresis
Author(s)
Felley-Bosco E., Demalte I., Barcelo S., Sanchez J.C., Hochstrasser D.F., Schlegel W., Reymond M.A.
ISSN
0173-0835
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
20
Number
18
Pages
3508-3513
Language
english
Abstract
To determine the feasibility of data transfer, an interlaboratory comparison was conducted on colon carcinoma cell line (DLD-1) proteins resolved by two-dimensional polyacrylamide gel electrophoresis either on small (6 x 7 cm) or large (16x18 cm) gels. The gels were silver-stained and scanned by laser densitometry, and the image obtained was analyzed using Melanie software. The number of spots detected was 1337+/-161 vs. 2382+/-176 for small vs. large format gels, respectively. After gel calibration using landmarks determined using pl and Mr markers, large- and small-format gels were matched and 712+/-36 proteins were found on both types of gels. Having performed accurate gel matching it was possible to acquire additional information after accessing a 2-D PAGE reference database (http://www.expasy.ch/ cgibin/map2/def?DLD1_HUMAN). Thus, the difference in gel size is not an obstacle for data transfer. This will facilitate exchanges between laboratories or consultation concerning existing databases.
Keywords
Adenocarcinoma/chemistry, Adenocarcinoma/pathology, Colorectal Neoplasms/chemistry, Colorectal Neoplasms/pathology, Electrophoresis, Gel, Two-Dimensional/methods, Humans, Tumor Cells, Cultured
Pubmed
Web of science
Create date
19/11/2007 12:01
Last modification date
20/08/2019 12:37
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