Energetics of RecA-mediated recombination reactions. Without ATP hydrolysis RecA can mediate polar strand exchange but is unable to recycle.

Details

Serval ID
serval:BIB_107728CBE58E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Energetics of RecA-mediated recombination reactions. Without ATP hydrolysis RecA can mediate polar strand exchange but is unable to recycle.
Journal
Journal of Molecular Biology
Author(s)
Rosselli W., Stasiak A.
ISSN
0022-2836[print], 0022-2836[linking]
Publication state
Published
Issued date
11/1990
Volume
216
Number
2
Pages
335-352
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We demonstrate that the step of DNA strand exchange during RecA-mediated recombination reaction can occur equally efficiently in the presence or absence of ATP hydrolysis. The polarity of strand exchange is the same when instead of ATP its non-hydrolyzable analog adenosine-5'-O-(3-thiotriphosphate) is used. We show that the ATP dependence of recombination reaction is limited to the post-exchange stages of the reactions. The low DNA affinity state of RecA protomers, induced after ATP hydrolysis, is necessary for the dissociation of RecA-DNA complexes at the end of the reaction. This dissociation of RecA from DNA is necessary for the release of recombinant DNA molecules from the complexes formed with RecA and for the recycling of RecA protomers for another round of the recombination reaction.
Keywords
Adenosine Triphosphate/analogs &amp, derivatives, Adenosine Triphosphate/metabolism, Bacteriophage phi X 174/genetics, Base Sequence, DNA, Circular/genetics, DNA, Circular/metabolism, DNA, Viral/genetics, DNA, Viral/metabolism, Escherichia coli/genetics, Escherichia coli/metabolism, Microscopy, Electron, Models, Genetic, Molecular Sequence Data, Oligonucleotide Probes, Protein Binding, Rec A Recombinases/metabolism, Rec A Recombinases/ultrastructure, Recombination, Genetic
Pubmed
Web of science
Create date
24/01/2008 10:36
Last modification date
20/08/2019 12:37
Usage data