Nonvectorial surface transport, endocytosis via a Di-leucine-based motif, and bidirectional transcytosis of chimera encoding the cytosolic tail of rat FcRn expressed in Madin-Darby canine kidney cells.

Details

Serval ID
serval:BIB_10492
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nonvectorial surface transport, endocytosis via a Di-leucine-based motif, and bidirectional transcytosis of chimera encoding the cytosolic tail of rat FcRn expressed in Madin-Darby canine kidney cells.
Journal
Journal of Biological Chemistry
Author(s)
Stefaner I., Praetor A., Hunziker W.
ISSN
0021-9258
Publication state
Published
Issued date
1999
Volume
274
Number
13
Pages
8998-9005
Language
english
Notes
Publication types: Journal Article
Abstract
Transfer of passive immunity from the mother to the fetus or newborn involves the transport of IgG across several epithelia. Depending on the species, IgG is transported prenatally across the placenta and yolk sac or is absorbed from colostrum and milk by the small intestine of the suckling newborn. In both cases apical to basolateral transepithelial transport of IgG is thought to be mediated by FcRn, an IgG Fc receptor with homology to major histocompatibility class I antigens. Here, we analyzed the intracellular routing of chimera encoding the rat FcRn tail fused to the ecto- and transmembrane domain of the macrophage FcgammaRIIb. Newly synthesized chimera were delivered in a nonvectorial manner to the apical and basolateral cell surface, from where the chimera were able to internalize and transcytose. Apical to basolateral and basolateral to apical transcytosis were differently regulated. This intracellular routing of the chimera is similar to that of the native FcRn, indicating that the cytosolic tail of the receptor is necessary and sufficient to endow an unrelated FcR with the intracellular transport behavior of FcRn. Furthermore, the di-leucine motif in the cytosolic domain of FcRn was required for rapid and efficient endocytosis but not for basolateral sorting of the chimera.
Keywords
Amino Acid Sequence, Animals, Antigens, CD/genetics, Antigens, CD/metabolism, Biological Transport/genetics, Cell Line, Dogs, Endocytosis/genetics, Histocompatibility Antigens Class I, Immunoglobulin Fab Fragments/analysis, Immunohistochemistry, Macrophages/metabolism, Molecular Sequence Data, Rats, Receptors, Fc/genetics, Receptors, Fc/metabolism, Receptors, IgG/genetics, Receptors, IgG/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Transfection/genetics
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:00
Last modification date
20/08/2019 12:37
Usage data