Modulation of human cytotrophoblastic leptin secretion by interleukin-1alpha and 17beta-oestradiol and its effect on HCG secretion

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Serval ID
serval:BIB_10204C7E853A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Modulation of human cytotrophoblastic leptin secretion by interleukin-1alpha and 17beta-oestradiol and its effect on HCG secretion
Journal
Molecular Human Reproduction
Author(s)
Chardonnens  D., Cameo  P., Aubert  M. L., Pralong  F. P., Islami  D., Campana  A., Gaillard  R. C., Bischof  P.
ISSN
1360-9947 (Print)
Publication state
Published
Issued date
11/1999
Volume
5
Number
11
Pages
1077-82
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
To investigate the role of leptin during pregnancy, we assessed leptin production by pure cultured human cytotrophoblastic cells (CTB), its regulation by cytokines and 17beta-oestradiol and its effects on human chorionic gonadotrophin (HCG) secretion. Purified CTB from first trimester placenta were incubated in duplicates in the presence or absence of cytokines or 17beta-oestradiol. Medium was harvested on day 2 and the culture stopped on day 4. Results were corrected for protein content of each individual well and expressed as percent of controls per day (mean +/- SEM). Basal CTB leptin production was 25.2 +/- 2.6 (ng/mg prot). In comparison with controls, leptin production was stimulated to 320 +/- 16% (P < 0.0001) and 195 +/- 3.2% (P < 0.0004) by 3 and 10 ng/ml of interleukin-1alpha respectively. 17beta-oestradiol 10(-6) to 10(-9) mol/l increased basal leptin production 5-9-fold, while 10(-5) mol/l had no such effect. Basal CTB HCG secretion was 5722 +/- 1055 (mIU/mg prot). There was a dose-dependent leptin-induced increase in HCG secretion (P = 0.0039) reaching a 5-fold increase with a leptin concentration of 1 microg/ml (P < 0.006). Gonadotrophin-releasing hormone (GnRH) 8.5 x 10(-8) mol/l significantly increased HCG secretion to 140 +/- 21% of controls (P = 0.031). Cetrorelix (0.1 microg/ml) inhibited leptin-induced HCG secretion (P = 0.0028).
Keywords
Cells, Cultured Chorionic Gonadotropin/*secretion Estradiol/*metabolism Female Gonadotropin-Releasing Hormone/antagonists & inhibitors/metabolism Humans Interleukin-1/*metabolism Leptin/metabolism/*secretion Pregnancy Recombinant Proteins/metabolism/secretion Trophoblasts/metabolism/*secretion
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:26
Last modification date
25/09/2019 6:08
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