Proliferation of chick embryo neuroblasts grown in the presence of horse serum requires exogenous transferrin.

Details

Serval ID
serval:BIB_0FF859A22670
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Proliferation of chick embryo neuroblasts grown in the presence of horse serum requires exogenous transferrin.
Journal
Journal of neuroscience research
Author(s)
Barakat-Walter I., Deloulme J.C., Sensenbrenner M., Labourdette G.
ISSN
0360-4012
Publication state
Published
Issued date
1991
Peer-reviewed
Oui
Volume
28
Number
3
Pages
391-398
Language
english
Abstract
We have previously shown that neuroblasts from cerebral hemispheres of 6-day-old chick embryos are able to proliferate when grown in the presence of fetal calf serum. We report here that in the presence of horse serum alone the proliferative rate of neuroblasts is strongly reduced. A high proliferative rate is restored upon the addition of bovine transferrin and to a lesser extent with added FeSO4 or hemin. These findings suggest that the transferrin of horse serum cannot be used by chick neuroblasts in vitro, while bovine transferrin exogenously added is active in promoting cell proliferation. We propose that the stimulatory activity of the fetal calf serum is due to bovine transferrin, since when this serum is fractionated by gel filtration, the fractions that stimulate the proliferation of neuroblasts grown in the presence of horse serum are located in the molecular weight area of transferrin, and they do contain transferrin as seen by immunoblotting with a specific anti-transferrin antibody.
Keywords
Animals, Blood Physiological Phenomena, Brain, Cattle, Cell Division, Cells, Cultured, Chick Embryo, Culture Media, Ferrous Compounds, Hemin, Horses, Neurons, Species Specificity, Stem Cells, Transferrin
Pubmed
Web of science
Create date
30/03/2009 8:31
Last modification date
20/08/2019 12:36
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