Expression of a functional single chain antibody on the surface of extracellular enveloped vaccinia virus as a step towards selective tumour cell targeting.

Details

Serval ID
serval:BIB_0FD79090F021
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expression of a functional single chain antibody on the surface of extracellular enveloped vaccinia virus as a step towards selective tumour cell targeting.
Journal
Journal of General Virology
Author(s)
Galmiche M.C., Rindisbacher L., Wels W., Wittek R., Buchegger F.
ISSN
0022-1317[print], 0022-1317[linking]
Publication state
Published
Issued date
11/1997
Volume
78 ( Pt 11)
Pages
3019-3027
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Recombinant vaccinia virus with tumour cell specificity may provide a versatile tool either for direct lysis of cancer cells or for the targeted transfer of genes encoding immunomodulatory molecules. We report the expression of a single chain antibody on the surface of extracellular enveloped vaccinia virus. The wild-type haemagglutinin, an envelope glycoprotein which is not required for viral infection and replication, was replaced by haemagglutinin fusion molecules carrying a single chain antibody directed against the tumour-associated antigen ErbB2. ErbB2 is an epidermal growth factor receptor-related tyrosine kinase overexpressed in a high percentage of human adenocarcinomas. Two fusion proteins carrying the single chain antibody at different NH2-terminal positions were expressed and exposed at the envelope of the corresponding recombinant viruses. The construct containing the antibody at the site of the immunoglobulin-like loop of the haemagglutinin was able to bind solubilized ErbB2. This is the first report of replacement of a vaccinia virus envelope protein by a specific recognition structure and represents a first step towards modifying the host cell tropism of the virus.
Keywords
Animals, Antibodies, Monoclonal/immunology, Antibodies, Monoclonal/therapeutic use, Antibodies, Viral/immunology, Antibodies, Viral/therapeutic use, DNA, Recombinant, Genetic Vectors, Humans, Neoplasms/drug therapy, Neoplasms/genetics, Vaccinia virus/genetics, Vaccinia virus/immunology, Viral Envelope Proteins/genetics, Viral Envelope Proteins/immunology
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 12:36
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