Adult-derived human liver mesenchymal-like cells as a potential progenitor reservoir of hepatocytes?

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State: Public
Version: Final published version
Serval ID
serval:BIB_0FCD43D65451
Type
Article: article from journal or magazin.
Collection
Publications
Title
Adult-derived human liver mesenchymal-like cells as a potential progenitor reservoir of hepatocytes?
Journal
Cell Transplantation
Author(s)
Najimi M., Khuu D.N., Lysy P.A., Jazouli N., Abarca J., Sempoux C., Sokal E.M.
ISSN
0963-6897 (Print)
ISSN-L
0963-6897
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
16
Number
7
Pages
717-728
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
It is currently accepted that adult tissues may develop and maintain their own stem cell pools. Because of their higher safety profile, adult stem cells may represent an ideal candidate cell source to be used for liver cell therapies. We therefore evaluated the differentiation potential of mesenchymal-like cells isolated from adult human livers. Mesenchymal-like cells were isolated from enzymatically digested adult human liver and expanded in vitro. Cell characterization was performed using flow cytometry, RT-PCR, and immunofluorescence, whereas the differentiation potential was evaluated both in vitro after incubation with specific media and in vivo after intrasplenic transplantation of uPA(+/+)-SCID and SCID mice. Adult-derived human liver mesenchymal-like cells expressed both hepatic and mesenchymal markers among which albumin, CYP3A4, vimentin, and alpha-smooth muscle actin. In vitro differentiation studies demonstrated that these mesenchymal-like cells are preferentially determined to differentiate into hepatocyte-like cells. Ten weeks following intrasplenic transplantation into uPA(+/+)-SCID mice, recipient livers showed the presence of human hepatocytic cell nodules positive for human albumin, prealbumin, and alpha-fetoprotein. In SCID transplanted liver mice, human hepatocyte-like cells were mostly found near vascular structures 56 days posttransplantation. In conclusion, the ability of isolated adult-derived liver mesenchymal stem-like cells to proliferate and differentiate into hepatocyte-like cells both in vitro and in vivo leads to propose them as an attractive expandable cell source for stem cell therapy in human liver diseases.
Keywords
Adult, Animals, Biomarkers/metabolism, Cell Differentiation/physiology, Cell Lineage, Cell Shape, Hepatocytes/cytology, Hepatocytes/physiology, Humans, Liver/cytology, Mesenchymal Stromal Cells/cytology, Mesenchymal Stromal Cells/physiology, Mice, Mice, SCID, Stem Cell Transplantation, Transplantation, Heterologous
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Web of science
Create date
20/10/2016 16:09
Last modification date
20/08/2019 12:36
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