Expression of connexin36 in the adult and developing rat brain

Details

Serval ID
serval:BIB_0FB7B5C81F61
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression of connexin36 in the adult and developing rat brain
Journal
Brain Research
Author(s)
Belluardo  N., Mudo  G., Trovato-Salinaro  A., Le Gurun  S., Charollais  A., Serre-Beinier  V., Amato  G., Haefliger  J. A., Meda  P., Condorelli  D. F.
ISSN
0006-8993 (Print)
Publication state
Published
Issued date
05/2000
Volume
865
Number
1
Pages
121-38
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 19
Abstract
The distribution of connexin36 (Cx36) in the adult rat brain and retina has been analysed at the protein (immunofluorescence) and mRNA (in situ hybridization) level. Cx36 immunoreactivity, consisting primarily of round or elongated puncta, is highly enriched in specific brain regions (inferior olive and the olfactory bulb), in the retina, in the anterior pituitary and in the pineal gland, in agreement with the high levels of Cx36 mRNA in the same regions. A lower density of immunoreactive puncta can be observed in several brain regions, where only scattered subpopulations of cells express Cx36 mRNA. By combining in situ hybridization for Cx36 mRNA with immunohistochemistry for a general neuronal marker (NeuN), we found that neuronal cells are responsible for the expression of Cx36 mRNA in inferior olive, cerebellum, striatum, hippocampus and cerebral cortex. Cx36 mRNA was also demonstrated in parvalbumin-containing GABAergic interneurons of cerebral cortex, striatum, hippocampus and cerebellar cortex. Analysis of developing brain further revealed that Cx36 reaches a peak of expression in the first two weeks of postnatal life, and decreases sharply during the third week. Moreover, in these early stages of postnatal development Cx36 is detectable in neuronal populations that are devoid of Cx36 mRNA at the adult stage. The developmental changes of Cx36 expression suggest a participation of this connexin in the extensive interneuronal coupling which takes place in several regions of the early postnatal brain.
Keywords
Age Factors Animals Animals, Newborn Biological Markers Brain/cytology/*growth & development/*metabolism Brain Mapping Connexins/*genetics/*metabolism Eye Proteins/*genetics/*metabolism Gap Junctions/metabolism Immunohistochemistry Male Neurons/cytology/*metabolism Nuclear Proteins/metabolism Parvalbumins/metabolism Pineal Gland/cytology/metabolism Pituitary Gland/cytology/metabolism RNA, Messenger/metabolism Rats
Pubmed
Web of science
Create date
25/01/2008 14:48
Last modification date
20/08/2019 13:36
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