Caspofungin activity against clinical isolates of azole cross-resistant Candida glabrata overexpressing efflux pump genes.

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Serval ID
serval:BIB_0FA69685624F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Caspofungin activity against clinical isolates of azole cross-resistant Candida glabrata overexpressing efflux pump genes.
Journal
The Journal of Antimicrobial Chemotherapy
Author(s)
Posteraro B., Sanguinetti M., Fiori B., La Sorda M., Spanu T., Sanglard D., Fadda G.
ISSN
0305-7453
Publication state
Published
Issued date
08/2006
Peer-reviewed
Oui
Volume
58
Number
2
Pages
458-461
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
OBJECTIVES: Several studies have documented the potent in vitro activity of caspofungin against Candida spp. This is of special concern for Candida glabrata infections that are often resistant to many azole antifungal agents and, consequently, difficult to treat. The aim of the present study was to expand the data on the in vitro activity of caspofungin against azole-resistant isolates of C. glabrata. METHODS: A total of 50 clinical isolates of C. glabrata were tested for susceptibility to caspofungin. The isolates were cross-resistant to multiple azoles, including fluconazole, itraconazole, ketoconazole and voriconazole. Expression of the resistance-related CgCDR1 and CgCDR2 genes was evaluated by quantitative RT-PCR analysis. The MICs of caspofungin were determined by using the National Committee for Clinical Laboratory Standards M27-A2 reference method. RESULTS: C. glabrata isolates exhibited increased expression of the CDR efflux pump(s), and this was in accordance with their high-level azole resistance. In contrast, all the isolates were highly susceptible to caspofungin (100% of isolates were inhibited at <or=1 mg/L). CONCLUSIONS: Our results represent further evidence for the excellent antifungal potency of caspofungin, particularly against C. glabrata isolates expressing cross-resistance to azoles.
Keywords
Antifungal Agents/pharmacology, Azoles/pharmacology, Biological Transport, Active/genetics, Candida glabrata/drug effects, Candida glabrata/genetics, Drug Resistance, Fungal/genetics, Echinocandins, Gene Expression, Humans, Microbial Sensitivity Tests, Peptides, Cyclic/pharmacology, RNA, Fungal/analysis, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:40
Last modification date
25/09/2019 6:08
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